| 镉与S-异丙甲草胺对斜生栅藻的联合毒性作用 |
| 摘要点击 1988 全文点击 1037 投稿时间：2014-09-09 修订日期：2014-11-03 |
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| 中文关键词 S-异丙甲草胺 Cd2+ 斜生栅藻 联合毒性 SOD酶活性 细胞膜通透性 |
| 英文关键词 S-metolachlor Cd2+ S. obliquus the combined toxicity SOD activity cell membrane permeability |
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| 中文摘要 |
| 采用毒性标准实验方法研究了Cd2+与S-异丙甲草胺单独及联合作用对斜生栅藻急性毒性、总可溶性蛋白含量、超氧化物歧化酶(SOD)活性和细胞膜通透性的影响. 结果表明Cd2+与S-异丙甲草胺单独作用时EC50均随时间的延长而减小,且S-异丙甲草胺的急性毒性大于Cd2+; Cd2+、S-异丙甲草胺的EC50-24h分别为0.27 mg·L-1、0.24 mg·L-1,EC50-96h分别为0.16 mg·L-1、0.13 mg·L-1. Cd2+与S-异丙甲草胺联合作用时低浓度表现为协同作用,高浓度表现为拮抗作用. 暴露96 h后,Cd2+与S-异丙甲草胺单独及联合作用下,随有毒物质浓度升高,斜生栅藻总可溶性蛋白含量降低,SOD酶活性先激活后抑制,细胞膜通透性逐渐增大. |
| 英文摘要 |
| The single and combined effects of Cd2+ and S-metolachlor on acute toxicity, total soluble protein content, superoxide dismutase (SOD) activity and cell membrane permeability of Scenedesmus obliquus (S. obliquus) were studied using the standard toxic testing methods. The results showed that the EC50 of Cd2+ and S-metolachlor decreased with time, and the acute toxicity of S-metolachlor was higher than that of Cd2+, EC50-24h of Cd2+ and S-metolachlor was 0.27 mg·L-1 and 0.24 mg·L-1, respectively, and EC50-96h was 0.16 mg·L-1 and 0.13 mg·L-1, respectively. The combined toxicity of Cd2+ and S-metolachlor showed a synergistic effects at low concentration, and antagonism effects at high concentration. After 96h-exposure, the total soluble protein content of S. obliquus decreased, the SOD activity first increased and then decreased, and the cell membrane permeability increased with the increasing concentration of both single and combined treatment. |
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