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黄河源典型流域内高寒草甸土壤微生物群落特征及其对土壤团聚体稳定性的影响
摘要点击 568  全文点击 13  投稿时间:2025-04-02  修订日期:2025-09-18
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中文关键词  黄河源流域  高寒草甸  微生物结构  微生物预测  土壤团聚体稳定性
英文关键词  Yellow River Source Region Watershed  alpine meadows  microbial community structure  microbial functional prediction  stability of soil aggregates
DOI  10.13227/j.hjkx.202504025
作者单位E-mail
张玉芳 青海大学省部共建三江源生态与高原农牧业国家重点实验室, 西宁 810016
青海大学农牧学院, 西宁 810016 
1249212374@qq.com 
李希来 青海大学省部共建三江源生态与高原农牧业国家重点实验室, 西宁 810016
青海大学农牧学院, 西宁 810016 
xilai-li@163.com 
张静 青海大学农牧学院, 西宁 810016 qhlxl2001@163.com 
鲍玉英 青海大学省部共建三江源生态与高原农牧业国家重点实验室, 西宁 810016
青海大学农牧学院, 西宁 810016 
 
周怡志 青海大学省部共建三江源生态与高原农牧业国家重点实验室, 西宁 810016
青海大学农牧学院, 西宁 810016 
 
柴瑜 青海大学省部共建三江源生态与高原农牧业国家重点实验室, 西宁 810016
青海大学农牧学院, 西宁 810016 
 
旦正卓玛 青海大学农牧学院, 西宁 810016  
韩玉兰 青海大学农牧学院, 西宁 810016  
马蕊 青海大学农牧学院, 西宁 810016  
安海宏 玛曲县草原工作站, 玛曲 747300  
中文摘要
      微生物是参与土壤团聚体形成的关键因素,但是从微生物功能预测角度分析土壤团聚体稳定性的研究较少. 因此,以黄河源典型流域上中下游高寒草甸为研究对象,通过分析其土壤理化性质和微生物群落组成,并结合真菌FUNGuild预测和细菌BugBase表型预测,旨在揭示流域单元内土壤团聚体稳定性的空间差异及其驱动因素. 结果表明:①土壤团聚体稳定性表现为:上游>下游>中游;②土壤细菌和真菌群落结构在上中下游差异显著(P<0.05),细菌主要优势种[放线菌门(Actinobacteriota)、变形菌门(Proteobacteria)、酸杆菌门(Acidobacteriota)]和真菌主要优势种[子囊菌门(Ascomycota)、担子菌门(Basidiomycota)、被孢霉门(Mortierellomycota)]相对丰度在流域上中下游无显著差异(P>0.05);③好氧和革兰氏阳性是土壤细菌最主要的两类表型,上游好氧类群平均相对丰度高于中游和下游,厌氧类群相对丰度低于中游和下游,细菌表型功能对土壤团聚体稳定性无显著影响(P>0.05);腐生菌是土壤真菌最主要的功能群,其中上游土壤木质腐生真菌相对丰度显著高于中游和下游;④流域单元内,土壤真菌群落通过影响真菌功能群组成来调控土壤团聚体稳定性.
英文摘要
      Microorganisms are key factors involved in soil aggregate formation; however, research analyzing soil aggregate stability from the perspective of microbial functional prediction remains limited. Therefore, this study selected alpine meadows along the upper, middle, and lower reaches of a typical watershed in the Yellow River source region as the research subject. By analyzing soil physicochemical properties and microbial community composition and combining Fungal FUNGuild prediction with Bacterial BugBase phenotype prediction, it aims to reveal the spatial variations in soil aggregate stability within the watershed unit and identify their driving factors. ① The stability of soil aggregates was upstream>downstream>midstream. ② There were significant differences in the community structure of soil bacteria and fungi upstream, midstream, and downstream (P<0.05), and the dominant bacterial species were Actinobacteriota, Proteobacteria, Acidobacteriota, Ascomycota, and Basidiomycota, and there was no significant difference in the relative abundance of Mortierellomycota in the upstream, midstream, and downstream (P<0.05). ③ The aerobic and gram positive phenotypes were the two most predominant bacterial groups in the soil. The average relative abundance of aerobic bacterial communities was higher in upstream regions compared to that in the midstream and downstream areas, while the relative abundance of anaerobic communities was lower in upstream regions than that in the midstream and downstream. Bacterial phenotypic functions showed no significant impact on soil aggregate stability(P>0.05). Saprotrophs constituted the dominant functional group of soil fungi, with the relative abundance of wood saprotrophic fungi in upstream soils being significantly higher than that in midstream and downstream soils. ④ In the watershed unit, the soil fungal community regulated the stability of soil aggregates by affecting the composition of fungal functional groups.

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