| 丛枝菌根真菌及黏土矿物对土壤微生物群落结构的影响 |
| 摘要点击 234 全文点击 6 投稿时间：2025-02-21 修订日期：2025-05-24 |
| 查看HTML全文
查看全文 查看/发表评论 下载PDF阅读器 |
| 中文关键词 丛枝菌根真菌（AMF） 黏土矿物 群落结构 土壤 细菌 |
| 英文关键词 arbuscular mycorrhizal fungi （AMF） clay minerals community structure soil bacteria |
| DOI 10.13227/j.hjkx.202502175 |
| 作者 | 单位 | E-mail | | 赵志瑞 | 河北地质大学水资源与环境学院, 河北省高校生态环境地质应用技术研发中心, 河北省水资源可持续利用与产业结构优化协同创新中心, 石家庄 050031 | zhiruizh@163.com | | 刘慧 | 河北地质大学水资源与环境学院, 河北省高校生态环境地质应用技术研发中心, 河北省水资源可持续利用与产业结构优化协同创新中心, 石家庄 050031 | | | 罗佳龙 | 河北地质大学水资源与环境学院, 河北省高校生态环境地质应用技术研发中心, 河北省水资源可持续利用与产业结构优化协同创新中心, 石家庄 050031 | | | 陈小刚 | 河北地质大学水资源与环境学院, 河北省高校生态环境地质应用技术研发中心, 河北省水资源可持续利用与产业结构优化协同创新中心, 石家庄 050031 | | | 李硕阳 | 河北地质大学水资源与环境学院, 河北省高校生态环境地质应用技术研发中心, 河北省水资源可持续利用与产业结构优化协同创新中心, 石家庄 050031 | | | 左天源 | 河北地质大学水资源与环境学院, 河北省高校生态环境地质应用技术研发中心, 河北省水资源可持续利用与产业结构优化协同创新中心, 石家庄 050031 | | | 宋泽峰 | 河北地质大学水资源与环境学院, 河北省高校生态环境地质应用技术研发中心, 河北省水资源可持续利用与产业结构优化协同创新中心, 石家庄 050031 | | | 栾卓然 | 河北地质大学水资源与环境学院, 河北省高校生态环境地质应用技术研发中心, 河北省水资源可持续利用与产业结构优化协同创新中心, 石家庄 050031 | 1033467796@qq.com | | 张超 | 河北地质大学水资源与环境学院, 河北省高校生态环境地质应用技术研发中心, 河北省水资源可持续利用与产业结构优化协同创新中心, 石家庄 050031
中国科学院生态环境研究中心, 北京 100085 | chaozhang@rcees.ac.cn |
|
| 中文摘要 |
| 目前丛枝菌根真菌（AMF）与黏土矿物对农田土壤的田间应用研究较少，研究了大田种植条件下AMF及黏土矿物对土壤微生物群落的影响，分别把AMF和黏土矿物施加到小麦种植土壤，采用高通量测序等技术研究添加剂对土壤细菌群落和真菌群落及理化指标等的影响. 结果表明，与空白相比，分别添加AMF和矿物后，降低了小麦根际土壤的碱性，单添加黏土矿物pH降低最多，降幅达到13.7%，与空白样品相比，其他3个样品中碱解氮（AN）都出现了增加，增幅分别为4.53%、6.1%和26.22%. 在细菌门水平上，与空白相比，分别添加和配施AMF菌剂和矿物，变形菌门生长条件得到优化，该菌相对丰度增加. 在细菌门水平上，与空白相比，添加AMF菌剂和矿物，酸杆菌门的丰度逐渐降低. 优势菌门与土壤肥力指标的相关性显示，变形菌门与碱解氮显著正相关，而与总氮呈负相关，变形菌门在利用土壤中氮素进行代谢活动时，对其他养分元素有一定的促进作用. 酸杆菌门与pH和总磷显著正相关，对有机质、碱解氮、速效钾和速效磷负相关. 放线菌门与有机质、速效钾和速效磷显著正相关，与总氮正相关，与pH、总磷和总钾负相关，与碱解氮相关性较低. 硬囊霉属与TP显著正相关，而与TN和TK正相关，近明球囊霉属与TK显著正相关. COG功能分类统计显示蛋白丰度趋势比较相似，但不同样品蛋白丰度发生了上调或下调. 研究结果可为提升土壤肥力与健康，农业可持续发展提供理论依据. |
| 英文摘要 |
| At present， there are few studies on the field application of arbuscular mycorrhizal fungi （AMF） and clay minerals on farmland soil. The effects of AMF and clay minerals on soil microbial community under field planting conditions were studied. AMF and clay minerals were applied to wheat planting soil， and high-throughput sequencing and other technologies were used to investigate the effects of additives on soil bacterial communities， fungal communities， and physicochemical indicators. The results showed that compared with the blank， the alkalinity of wheat rhizosphere soil was reduced after the addition of AMF and minerals respectively， and the pH of clay minerals alone decreased the most， with a decrease of 13.7%， and compared with the blank sample， the alkaline hydrolyzable nitrogen （AN） in the other three samples increased by 4.53%， 6.1%， and 26.22%， respectively. At the bacterial phylum level， compared with the blank， the growth conditions of Proteobacteria were optimized and the relative abundance of the proteobacteria phylum was increased by the addition and application of AMF agents and minerals respectively. At the level of bacterial phylum， the abundance of Acidobacteriota was progressively reduced with the addition of AMF agents and minerals compared to the blank. The correlation between dominant bacteria and soil fertility indexes showed that Proteobacteria were significantly positively correlated with alkali hydrolyzable nitrogen， but negatively correlated with total nitrogen， and Proteobacteria had a certain promotion effect on other nutrients when using nitrogen in soil for metabolic activities. Acidobacteria was positively correlated with pH and total phosphorus， and negatively correlated with organic matter， alkaline hydrolyzable nitrogen， available potassium and available phosphorus. Actinomycetes were significantly positively correlated with organic matter， available potassium and available phosphorus， positively correlated with total nitrogen， negatively correlated with pH， total phosphorus and total potassium， and had a low correlation with alkaline hydrolyzable nitrogen. Sclerocystis was significantly positively correlated with total phosphorus and alkaline hydrolyzable nitrogen， positively correlated with total nitrogen and total potassium， while Claroideoglomus was significantly positively correlated with total potassium. The COG functional classification statistics showed that the protein abundance trend was similar， but the protein abundance of different samples was up-regulated or down-regulated. The results of this study can provide a theoretical basis for improving soil fertility and health and sustainable agricultural development. |
|
|
|
