| 不同灌丛根际土壤和根内生细菌的群落结构特征 |
| 摘要点击 2207 全文点击 378 投稿时间：2023-11-24 修订日期：2024-02-02 |
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| 中文关键词 灌丛 根际土壤细菌 根内生细菌 群落结构 高通量测序 |
| 英文关键词 shrub rhizosphere soil bacteria root-endophytic bacteria community structure high-throughput sequencing |
| DOI 10.13227/j.hjkx.20241152 |
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| 中文摘要 |
| 生态恢复过程中,植被在恢复生态系统功能方面发挥着重要作用,土壤微生物作为土壤生态系统的重要组成部分,不仅驱动了土壤的物质循环过程,而且提高了植物的生产力和抗逆性. 为明确不同灌丛根际土壤和根内生细菌的群落结构特征,利用Illumina高通量测序技术测定了晋西北黄土丘陵区的不同灌丛（灰栒子、忍冬和红瑞木）根际土壤和根内生细菌的组成.结果表明,不同灌丛根际土壤和根内生细菌的优势菌门均为变形菌门（Proteobacteria）和放线菌门（Actinobacteria）；不同灌丛根内生细菌共有的优势属有3个,灌丛的根际土壤和根内生细菌优势属类群不同.根际土壤细菌的物种丰富度和多样性指数均显著高于根内生细菌（P < 0.05）.灌丛中约64%的根内生细菌都存在根际土壤细菌中,所以不同生态位的细菌群落组成具有相似性.冗余分析和Pearson相关性分析表明,土壤脱氢酶、土壤N-乙酰-β-D葡萄糖苷酶、碱性蛋白酶、pH和总磷对根内生细菌群落组成影响显著（P < 0.05）,而碱性蛋白酶、pH、总碳和总氮对根际土壤细菌群落影响显著（P < 0.05）.偏最小二乘路径分析模型表明,植被能够直接影响细菌群落,也能通过影响土壤理化性质和土壤酶活性间接影响细菌群落.研究结果为进一步研究灌丛内生菌和抗逆性之间的关系提供一定理论依据. |
| 英文摘要 |
| In the process of ecological restoration, vegetation plays a crucial role in restoring ecosystem functions. Soil microorganisms are essential components of soil ecosystems, driving material cycling processes and enhancing plant productivity and resilience. This study aimed to investigate the community structure characteristics of rhizosphere soil and root-endophytic bacteria in different shrubs. Specifically, the composition of rhizosphere soil and root-endophytic bacteria in Cotoneaster acutifolius Turcz., Lonicera japonica Thunb., and Cornus alba L. in the loess hilly area of northwest Shanxi was determined using Illumina high-throughput sequencing technology. The results revealed that the dominant phyla of rhizosphere soil bacteria and root-endophytic bacteria in different shrubs were Proteobacteria and Actinobacteria. Additionally, the genera of rhizosphere soil and root-endophytic bacteria differed. Furthermore, the species richness and diversity index of rhizosphere soil bacteria were significantly higher than those of root-endophytic bacteria （P < 0.05）. It was also observed that approximately 64% of the root-endophytic bacteria in the shrubs were present in the rhizosphere soil bacteria, indicating similarity in the bacterial community compositions of different niches. Redundancy analysis （RDA） and Pearson correlation analysis revealed that soil dehydrogenase, soil N-acetyl-β-D glucosidase, alkaline protease, pH, and total phosphorus were the main influencing factors on the bacterial community structures in root-endophytic bacteria （P < 0.05）, while alkaline protease, pH, total carbon, and total nitrogen significantly impacted rhizosphere soil community structures （P < 0.05）. Additionally, the partial least squares path model （PLS-PM） indicated that vegetation could directly affect bacterial communities and indirectly affect them by influencing soil physicochemical properties and soil enzyme activity. In conclusion, the findings of this study provide a theoretical foundation for further research on the relationship between endophytic bacteria and resistance in shrubs. |
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