| 乌梁素海东部流域非生长季草地土壤细菌群落结构的垂向差异 |
| 摘要点击 1206 全文点击 315 投稿时间：2022-06-13 修订日期：2022-09-02 |
| 查看HTML全文
查看全文 查看/发表评论 下载PDF阅读器 |
| 中文关键词 草地 非生长季 细菌群落 空间差异 乌梁素海东部流域 |
| 英文关键词 grassland non-growing season bacterial community spatial difference eastern Ulansuhai Basin |
|
| 中文摘要 |
| 草地是区域生态系统的关键组成部分,其土壤细菌群落结构在元素迁移及生态多样性系统中发挥着重要作用.为对比分析寒旱区典型草地土壤中细菌群落的空间结构差异,在5月初(新的生长季开始之前,即非生长季),对内蒙古乌梁素海东部流域草地土壤样品进行了采集,获得不同区域30 cm和60 cm深度土壤样品各5个,利用16S rRNA高通量测序方法对样品细菌群落结构进行了分析.结果显示:①Actinobacteriota、Proteobacteria、Chloroflexi、Acidobacteriota、Gemmatimonadota、Planctomycetota、Methylomirabilota和Crenarchacota等菌门在30 cm和60 cm样品中相对丰度占比均超过1%,且60 cm样品中相对丰度高于30 cm样品的优势菌门有6个,优势菌属有5个,OTU则为8个.不同深度样品中优势菌门、菌属乃至OTUs的相对丰度变化和其在细菌群落结构中的贡献性趋势并不完全对应;②来源于Armatimonadota和Verrucomicrobiota门的norank_f__norank_o__norank_c__norank_p__Armatimonadota和Candidatus_Xiphinematobacter属分别在30 cm和60 cm样品中具有明显的细菌群落结构贡献度,可以作为土壤细菌群落结构垂向变化对比的关键细菌进行生态学分析;③和30 cm样品相比较,60 cm样品中与C、N和P元素代谢有关的ko00190、ko00910和ko01200功能相对丰度均出现一定程度的增加,显示代谢功能相对丰度的增加促进了草地土壤中C、N和P等典型营养元素的相对含量的降低,这将为开展草地土壤细菌群落的关键生态功能空间变化提供参考. |
| 英文摘要 |
| Grassland is an important part of the regional ecosystem, and its micro ecological structures play key roles in the process of element migration and the evolution of ecological diversity systems. To discover the spatial difference of the grassland soil bacterial community, we collected five total soil samples at 30 cm depth and 60 cm depth in Eastern Ulansuhai Basin in early May (before the beginning of the new growing season, with a minimum influence of human activities and other factors). Based on 16S rRNA gene-based high-throughput sequencing technology, the vertical characteristic of the bacterial community was analyzed in detail. First, Actinobacteriota, Proteobacteria, Chloroflexi, Acidobacteriota, Gemmatimonadota, Planctomycetota, Methylomirabilota, and Crenarchacota all appeared in the 30 cm and 60 cm samples, with the relative contents all being higher than 1%. In addition, there were a total of six phyla, five genera, and eight OTUs in the 60 cm sample with relative contents higher than those in the 30 cm sample. As a result, the relative abundance changes in dominant bacterial phyla, genera, and even OTUs at different sample depths did not correspond to their contribution to the bacterial community structure. Second, because of the unique contribution to the bacterial community structure in 30 cm and 60 cm samples, the norank_f__norank_o__norank_c__norank_p__Armatimonadota and Candidatus_Xiphinematobacter could be utilized as key bacterial genera during ecological system analysis, belonging to the Armatimonadota and Verrucomicrobiota, respectively. Finally, the relative abundances of ko00190, ko00910, and ko01200 were all higher in 60 cm samples than those in 30 cm samples, which showed that through the increase in metabolic function abundance, the relative contents of C, N, and P elements in grassland soil had been reduced with the increase in depth. These results will provide references for further study on the spatial change of bacterial communities in typical grassland. |
|
|
|
