| 调蓄水库细菌群落季节变化特征与影响因素 |
| 摘要点击 2395 全文点击 871 投稿时间：2022-04-06 修订日期：2022-06-05 |
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| 中文关键词 调蓄水库 细菌群落结构 季节变化 影响因素 |
| 英文关键词 storage reservoir bacterial community structure seasonal variation influencing factors |
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| 中文摘要 |
| 为探究调蓄水库水体细菌群落的季节变化特征与影响因素.以引黄济青工程的唯一调蓄水库——青岛棘洪滩水库为研究对象，采用高通量测序技术，结合水质分析指标，探究细菌群落结构以及与环境因子的关系.结果表明，细菌群落多样性和丰富度夏季最高，冬季最低，进水口高于出水口.细菌群落结构组成春季与冬季相似，夏季与秋季相似.Actinobacteriota （6.63%~57.38%）、Proteobacteria （11.32%~48.60%）、Bacteroidota （5.05%~25.74%）和Cyanobacteria （0.65%~24.74%）为优势菌门，Chloroflexi、Dependentiae、Fusobacteriota和Margulisbacteria相对丰度秋季最高，冬季最低；Patescibacteria和Myxococcota相对丰度夏季最高，冬季最低；Acidobacteria相对丰度春季最高，冬季最低；hgcI_clade（3.72%~34.66%）、CL 500_29_marine_group（0.31%~20.13%）和Limnohabitans（0.16%~10.37%）为优势菌属，Flavobacterium、Polaromonas和Rhodoferax相对丰度冬季最高，夏季最低；Domibacillus和Limnobacter相反.进水口Proteobacteria和Campilobacterota相对丰度明显高于出水口，Planctomycetota与之相反；进水口Dinghuibacter、Arenimonas和Rhodobacter相对丰度明显高于出水口.细菌群落互作关系春季以竞争和拮抗为主，冬季以互利共生为主，不同季节和采样点共生网络中关键物种存在显著差异.水温、DO、蓄水量和蓄水来源对棘洪滩水库细菌群落结构组成影响较大. |
| 英文摘要 |
| In order to explore the seasonal variation and influencing factors of bacterial community structure in storage reservoirs, the impact of environmental factors must first be examined. In this study, the seasonal variation in bacterial community structure and its response to water quality factors were explored by monitoring the water quality of Qingdao Jihongtan Reservoir, the only reservoir of the Yellow River diversion project, using high-throughput sequencing technology and symbiotic network analysis. The results showed that the diversity and richness of bacterial communities were highest in summer and lowest in winter, and those in the inlet were higher than those in the outlet. The structure of the bacterial community was similar in spring and winter and in summer to autumn. The dominant bacteria phyla were:Actinobacteriota (6.63%-57.38%), Proteobacteria (11.32%-48.60%), Bacteroidota (5.05%-25.74%), and Cyanobacteria (0.65%-24.74%). Additionally, the abundances of Chloroflexi, Dependentiae, Fusobacteriota, and Margulisbacteria were the highest in autumn and the lowest in winter. The dominant bacterial genera were:hgcI_clade (3.72%-34.66%), CL500_29_marine_group (0.31%-20.13%), and Limnohabitans (0.16%-10.37%). Further, the abundances of Flavobacterium, Polaromonas, and Rhodoferax were the highest in winter and the lowest in summer; the trend of Domibacillus and Limnobacter was the opposite. The abundance of Proteobacteria and Campilobacteria in the inlet was significantly higher than that in the outlet, and the Planctomycetota showed the opposite. The abundances of Dinghuibacter, Arenimonas, and Rhodobacter in the inlet were significantly higher than those in the outlet. Competition and antagonism dominated the interaction relationship of bacterial communities in spring, whereas mutualism dominated in winter. There were significant differences among key species in the symbiotic network at different seasons and sampling sites. Water temperature, DO, water storage capacity, and water storage sources had a great influence on bacterial community structure in the Jihongtan Reservoir. |
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