| 西安市典型景观水体水质及反硝化细菌种群结构 |
| 摘要点击 4028 全文点击 1154 投稿时间：2017-03-20 修订日期：2017-06-09 |
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| 中文关键词 景观水体 水质 nirS型反硝化细菌 Illumina高通量测序 |
| 英文关键词 scenic water water quality nirS-Type denitrifier community Illumina high throughput sequence |
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| 中文摘要 |
| 选取西安市区6个典型景观水体作为研究对象，在分析景观水体水质指标的基础上，运用Illumina高通量测序技术对景观水体中的nirS型反硝化细菌种群结构和多样性进行诊断，以期探明景观微污染水体水质与反硝化细菌种群结构多样性的偶联机制.结果表明，丰庆公园和劳动公园水体属于劣V类水，而曲江池、木塔寺生态遗址公园、新纪元公园水体符合V类水质标准，永阳公园水体符合Ⅳ类水标准.总氮从1.21~6.50 mg·L-1（P<0.05），永阳公园水体总氮最低，丰庆公园最高.劳动公园水体总磷含量最高，为0.10 mg·L-1，曲江池总磷最低，为0.02 mg·L-1（P<0.05）.劳动公园水体氨氮为曲江池和丰庆公园的4.44倍（P<0.01）.Illumina高通量测序结果表明6个景观水体中nirS型反硝化细菌优势种属差异显著，副球菌属（Paracoccus sp.）、假单胞菌属（Pseudomonas sp.）、红长命菌属（Rubrivivax sp.）为优势菌群.主成分分析（PCA）表明来自新纪元公园和木塔寺公园水体的nirS型反硝化细菌种群结构主要受水体NH4+-N和高锰酸盐指数含量的调控，而NO3--N、NO2--N、TN、TP和DO对劳动公园和丰庆公园水体的nirS型反硝化细菌的种群结构影响显著；水体pH对永阳公园的nirS型反硝化细菌的种群结构影响显著.结果表明，西安市典型景观水体nirS型反硝化细菌的种群结构受不同水质指标的综合调控. |
| 英文摘要 |
| Six micro-polluted landscape water bodies were selected to explore the relationship between water quality and the nirS-Type denitrifier community structure and diversity with an Illumina high-throughput sequencing technique. The results of a physico-chemical analysis of the water bodies showed that the Fengqing Park (FQ) and Laodong Park (LD) water bodies were classified as inferior Ⅴ water quality, the Qujiang Park (QJ), Mutasi Park (MTS), and Xinjiyuan Park (XJY) water bodies were classified as Ⅴ water quality, and the Yongyang Park (YY) water quality was classified as Ⅳ. The TN values varied from 1.21 mg·L-1 to 6.50 mg·L-1, with the lowest value found in YY and the highest in FQ (P<0.05). TP was significantly higher in LD (0.10 mg·L-1) and significantly lower in QJ[0.02 mg·L-1 (P<0.05)]. The NH4+-N in LD was 4.44 times higher than that in QJ and FQ (P<0.01). Illumina high-throughput sequencing revealed that the denitrifier community composition was significantly different among the six water bodies, and Paracoccus sp., Pseudomonas sp., and Rubrivivax sp. were the dominant genus species. A principle component analysis (PCA) indicated that the nirS-Type denitrifier communities of XJY and MTS were mainly regulated by NH4+-N and the permanganate index, LD and FQ were significantly influenced by NO3--N, NO2--N, TN, TP, and DO, and YY was significantly influenced by pH value. Our results showed that nirS-Type denitrifier communities were regulated by different water quality indicators. |
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