| 沼液SBR处理出水养殖螺旋藻 |
| 摘要点击 3006 全文点击 963 投稿时间：2016-12-19 修订日期：2017-01-20 |
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| 中文关键词 养猪沼液 螺旋藻 SBR 循环利用 氨氮 |
| 英文关键词 digested piggery wastewater(DPW) Spirulina platensis sequencing batch reactor(SBR) recycle ammonium |
| 作者 | 单位 | E-mail | | 蔡小波 | 常州大学环境与安全工程学院, 常州 213164
浙江清华长三角研究院生态环境研究所, 浙江省水质科学与技术重点实验室, 嘉兴 314006 | 664700919@qq.com | | 郁强强 | 浙江清华长三角研究院生态环境研究所, 浙江省水质科学与技术重点实验室, 嘉兴 314006 | | | 刘锐 | 浙江清华长三角研究院生态环境研究所, 浙江省水质科学与技术重点实验室, 嘉兴 314006 | liuruitsinghuazj@gmail.com | | 赵远 | 常州大学环境与安全工程学院, 常州 213164 | | | 陈吕军 | 浙江清华长三角研究院生态环境研究所, 浙江省水质科学与技术重点实验室, 嘉兴 314006
清华大学环境学院, 北京 100084 | |
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| 中文摘要 |
| 养猪沼液氮磷等营养物质丰富，可作为廉价的螺旋藻培养基，但其成分复杂，尤其是高氨氮等因素严重抑制螺旋藻的生长.采用序批式生物反应器（sequencing batch reactor， SBR）降低沼液中的氨氮浓度，通过改变进水中化学需氧量（chemical oxygen demand， COD）与总氮（total nitrogen， TN）的比值，研究了沼液中的亚硝态氮及硝态氮的保留情况，为螺旋藻生长提供氮源.通过对比螺旋藻在不同工况出水中的生长情况，以及氮元素的保留情况，筛选出最佳SBR工况.摇瓶试验结果表明，当进水COD/TN=3.0，出水中氨氮、硝态氮、亚硝态氮浓度分别为51.2、91.6、213.1 mg·L-1，此时螺旋藻具有较快生长速率，产率达到0.084 g·（L·d）-1.在此基础之上，通过放大螺旋藻培养规模至120L，研究了螺旋藻在室外大棚中的生长情况及螺旋藻对沼液中氮、磷元素的去除，结果表明螺旋藻在室外依然生长良好，培养10 d后，产率为（0.075±0.003）g·（L·d）-1，螺旋藻蛋白含量达到60%左右，养殖出水中氨氮去除率达到99%. |
| 英文摘要 |
| Digested piggery wastewater(DPW) contains high concentrations of nitrogen and phosphorus which could be used as a cost-effective culture medium for Spirulina platensis. However, Spirulina platensis would be limited by many factors in the complex composition of DPW, especially the high concentration of ammonium. In this paper, a traditional sequencing batch reactor(SBR) was used to remove these inhibitors in DPW. The retention of nitrate and nitrite in the effluent, which was used as nitrogen source for cultivating Spirulina platensis, was studied at different ratios of chemical oxygen demand(COD) to total nitrogen(TN) in the influent. By comparing the growth of Spirulina platensis in the related effluents, the operation condition of SBR was optimized. The lab-scale cultivation results showed that Spirulina platensis possessed a high biomass yield of 0.084 g·(L·d)-1 in the effluent when the COD/TN ratio of SBR influent was 3.0. In particular, the concentrations of ammonium, nitrate and nitrite in the effluent were 51.2 mg·L-1, 91.6 mg·L-1and 213.1 mg·L-1, respectively. Furthermore, the aforementioned effluent was also used to culture Spirulina platensis in a 120 L outdoor raceway pond, and the growth rate of Spirulina platensis reached(0.075±0.003)g·(L·d)-1 after 10-day culture. The protein content of Spirulina platensis was approximately 60% and the removal efficiency of ammonium was 99%. This study provides an alternative method for the utilization of DPW. |
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