| 城市污水中硝化菌群落结构与性能分析 |
| 摘要点击 3850 全文点击 1350 投稿时间：2016-05-04 修订日期：2016-05-30 |
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| 中文关键词 城市污水 硝化菌 群落结构 硝化性能 连续接种 |
| 英文关键词 raw sewage nitrobacteria community structure nitrification performance continuous seeding |
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| 中文摘要 |
| 对西安市第二和第三污水处理厂进水中硝化菌群落结构与性能进行调查分析.荧光原位杂交结果发现，进水中氨氧化菌（AOB）优势菌为Nitrosomonas europaea/Nitrosococcus mobilis lineage；亚硝酸盐氧化菌（NOB）的优势菌均为Nitrospira，次优势菌为Nitrobacter，且与Nitrococcus、Nitrospina并存.二污及三污进水中硝化菌个数占总细菌数（AOB+NOB）/EUB的平均个数百分比分别为（5.35±2.1）%和（6.0±2.8）%；在曝气2~16h后，活性基本恢复，最大氨氧化速率分别为（0.32±0.12）mg·（L·h）-1和（0.43±0.17）mg·（L·h）-1，亚硝酸盐氧化速率为（0.71±0.18）mg·（L·h）-1和（0.58±0.27）mg·（L·h）-1.因此，城市污水中含有活性硝化菌，对活性污泥系统有自然的连续接种作用，根据进水及活性污泥中硝化活性可以估算出城市污水中AOB与NOB对活性污泥的连续接种强度分别为0.08~0.09 g·（g·d）-1和0.11~0.24 g·（g·d）-1. |
| 英文摘要 |
| The communities and activity of nitrifiers collected from the raw sewage of the 2nd and 3rd wastewater treatment plants (WWTP) in Xi'an were investigated. FISH results indicated that the (AOB+NOB)/EUB percentages were (5.35±2.1)% and(6.0±2.8)% in the 2nd and 3rd WWTP, respectively. The dominant AOB was Nitrosomonas europaea/Nitrosococcus mobilis lineage and the dominant NOB was Nitrospira, the sub-dominant NOB was Nitrobacter, and coexisted with Nitrococcus, Nitrospina. Respirometric assays showed that the influent nitrifiers were active following a 2-16 hour period of metabolic induction. The ammonium utilized rate was (0.32±0.12) mg·(L·h)-1 and (0.43±0.17) mg·(L·h)-1, nitrite utilized rate was (0.71±0.18) mg·(L·h)-1 and (0.58±0.27) mg·(L·h)-1 for nitrifers in raw sewage fed to the 2nd and 3rd WWTP, respectively. Therefore, nitrifiers were present and active in the raw sewage, and played the role of natural continuous seeding in the activated sludge system. Based on the nitrification activity, the estimated continuous seeding intensity of AOB and NOB was 0.08-0.09 g·(g·d)-1 and 0.11-0.24 g·(g·d)-1, respectively. |
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