| 低DO下AGS-SBR处理低COD/N生活污水长期运行特征及种群分析 |
| 摘要点击 3737 全文点击 1614 投稿时间：2015-11-18 修订日期：2016-01-06 |
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| 中文关键词 高通量测序 菌群结构 同步脱氮除磷 低COD/N比 低溶解氧 |
| 英文关键词 high-throughput sequencing bacterial community structure simultaneous denitrification and phosphorus removal low COD/N ratio low DO |
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| 中文摘要 |
| 本研究在序批式活性污泥反应器(SBR)中接种好氧颗粒污泥(AGS),构成AGS-SBR系统,研究其在低DO(0.5~1.0 mg·L-1)条件下,处理低COD/N比(<4.0)生活污水同步脱氮除磷的长期稳定运行特性,并解析反应器的主要菌群构成.结果表明,在反应器运行的180d里,AGS-SBR系统表现出了良好且稳定的除污能力,反应器对水体中COD、NH4+-N、TN和TP平均去除率分别达到87.17%、95.21%、77.05%和91.11%.好氧颗粒污泥沉降性能一直很好,污泥始终保持着完整的颗粒外观和密实紧凑的结构,并没有出现明显的颗粒污泥解体的现象.同时,高通量测序结果表明,变形菌门、厚壁菌门、绿菌门、绿弯菌门和拟杆菌门为SBR-AGS反应器中主要优势菌群. Denitratisoma、Planctomycetaceae、Thauera、Comamonas、Nitrosomonas和Nitrospira是反应器中与脱氮有关菌群; Clostridium和Anaerolinea是除磷相关细菌. |
| 英文摘要 |
| This study utilized the sequencing batch activated sludge reactor (SBR) inoculated aerobic granular sludge (AGS) to treat the low COD/N ratio (<4.0) domestic wastewater under low DO (0.5-1.0 mg·L-1) concentration condition. Long-term performance of simultaneous nitrogen and phosphorus removal and bacterial community composition of AGS-SBR were studied. The results showed that the AGS-SBR system had good and stable decontamination abilities in its 180-day operation. The average removal rates of COD, NH4+-N, TN and TP were 87.17%, 95.21%, 77.05%, and 91.11%, respectively. At the same time, the AGS showed good settling performance, and always kept its integrated and compact structure. No obvious granular sludge disintegration phenomenon occurred in 180 days. Meanwhile, by using Illumina 16S rRNA gene MiSeq sequencing, we investigated the bacterial abundance in AGS-SBR reactor. Proteobacteria, Firmicutes, Chlorobi, Chloroflex, and Bacteroidetes were the dominant microbial communities in the simultaneous nitrogen and phosphorus removal reactor. Denitratisoma, Planctomycetaceae, Thauera, Comamonas, Nitrosomonas and Nitrospira were suggested to be the primary organisms responsible for the nitrogen removal. Clostridium and Anaerolinea were the main bacterial communities of phosphorus removal. |
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