| 萱草修复石油烃污染土壤的根际机制和根系代谢组学分析 |
| 摘要点击 2763 全文点击 1587 投稿时间：2015-10-04 修订日期：2015-11-15 |
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| 中文关键词 石油烃 污染土壤 萱草 根际机制 根系 代谢组学分析 |
| 英文关键词 total petroleum hydrocarbons (TPHs) contaminated soil Hemerocallis middendorfii Trautv. et Mey. rhizospheric mechanism root metabonomic analyses |
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| 中文摘要 |
| 采用温室盆栽方法,研究花卉植物萱草(Hemerocallis middendorfii Trautv.et Mey.)对大港油田石油烃污染土壤的修复,设定的土壤石油烃污染含量为: 0、10000和40000 mg·kg-1.结果表明,萱草对石油烃含量≤40000 mg·kg-1具有良好的耐性,并且萱草对石油烃污染土壤中石油烃的修复效果比较显著,主要表现在试验组石油烃的去除率分别为53.7%和33.4%,显著高于空白对照组(31.8%和12.0%).通过GC-MS测定土壤中的氨基酸、有机酸以及糖类等成分的相对含量,并结合PCA和PLS-DA模型探讨了土壤石油烃去除的根际机制.结果发现,萱草的种植确实改变了土壤各成分的分布特征,而且其中喃葡萄糖对石油烃的去除起到关键的作用.此外,对萱草根系代谢组学的分析结果显示,仅在污染组发现了特殊的代谢物丙氨酸、肉豆蔻酸、棕榈酸和亚油酸.而且,石油烃的暴露确实改变了萱草根系的初生代谢流,引起了一些代谢物的显著变化.总之,萱草可以种植于石油烃含量≤40000 mg·kg-1的污染土壤,并具备了对石油烃的修复能力; 同时石油烃的暴露改变了萱草的根系代谢,而这种改变可能是萱草对石油烃污染土壤做出的代谢响应. |
| 英文摘要 |
| The effects of a special ornamental plant Hemerocallis middendorfii Trautv. et Mey. on remediating petroleum-contaminated soil from the Dagang Oilfield in Tianjin, China, was studied by a greenhouse pot-culture experiment and the gradients of TPHs were 0, 10000 and 40000 mg·kg-1. The results suggested that H. middendorfii had a high tolerance to TPHs(≤40000 mg·kg-1). And H. middendorfii significantly(P<0.05)promoted the removal rate of TPHs (53.7% and 33.4%) compared with corresponding controls (31.8% and 12.0%) by natural degradation, respectively. The relative abundance of amino acids, organic acids and sugars and others in soil were analyzed by gas chromatography-mass spectrometry (GC-MS), and PCA and PLS-DA models were to investigate the rhizospheric mechanisms. The results suggested that H. middendorfii changed the distribution characteristics of each component in soil, and the glucopyranoside played a key role in the removal of TPHs. Furthermore, the results about comparative metabolic profile showed that some special metabolites were only found in the contaminated groups, including alanine, tetradecanoic acid, hexadecanoic acid and 9,12-octadecadienoic acid. Additionally, the exposure of TPHs changed the primary metabolic flux of roots, and caused the significant (P<0.01)change of metabolites. In conclusion, H. middendorfii might be an enduring ornamental plant for effective remediating TPHs (≤40000 mg·kg-1) in soil. But the exposure of TPHs had changed the metabolic profile of H. middendorfii in roots, which might be the metabolic response of H. middendorfii to petroleum-contaminated soil. |
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