| 温度对嗜酸性硫杆菌活性和生物成因次生铁矿物形成的影响 |
| 摘要点击 2445 全文点击 1867 投稿时间：2012-11-11 修订日期：2012-12-24 |
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| 中文关键词 温度 嗜酸性氧化亚铁硫杆菌 活性 次生铁矿物 |
| 英文关键词 temperature Acidithiobacillus ferrooxidan activity secondary iron mineral |
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| 中文摘要 |
| 通过实验室摇瓶试验研究了温度对嗜酸性氧化亚铁硫杆菌(Acidithiobacillus ferrooxidan)氧化亚铁的活性及对生物成因次生铁矿物形成的影响. 结果表明,在FeSO4-H2O-A.ferrooxidans休止细胞体系中,低温明显抑制了A.ferrooxidans菌的氧化活性. 培养5 d后,10℃和28℃体系中Fe2+氧化率分别为11.81%和100%. 当温度从10℃ (培养7 d)调至28℃后,Fe2+氧化率在1 d内就迅速上升到95.10%. Fe2+最高氧化速率依次为:10℃(培养 7 d)+28℃(2.25 h-1)>28℃(1.42 h-1)>10℃(0.81 h-1). 次生铁矿物XRD图谱表明,在9K培养基中,10℃条件下较低的Fe3+供应速率更有利于无定型施威特曼石的形成. 而在28℃条件下,施威特曼石优先于黄铁矾生成,且随着时间延长黄铁矾结晶度愈来愈高. 通过XRD图谱和SEM微形貌特征判断,28℃下生物合成次生铁矿物主要为施威特曼石和黄钾铁矾的混合物. |
| 英文摘要 |
| In this study, batch experiments were performed to investigate the effect of temperature on the Fe (Ⅱ) oxidation and the formation of biogenic secondary iron minerals by Acidithiobacillus ferrooxidan. Results showed that the low temperature significantly inhibited the oxidation activity of A. ferrooxidan. In the FeSO4-H2O biological oxidation system facilitated by A. ferrooxidan, it was found that after 5 days culture, the oxidation rates of Fe (Ⅱ) in treatments of 10℃ and 28℃ were 11.81% and 100%, respectively. In addition, it rapidly rose to 95.10% when the temperature was adjusted from 10℃ (cultured for 7 days) to 28℃ in 1 day, and the maximum oxidation rates were as follows: 10℃ (cultured for 7 days)+28℃ (2.25 h-1)>28℃ (1.42 h-1)>10℃ (0.81 h-1). Furthermore, the XRD patterns showed that the lower Fe (Ⅲ) supply rate was more conducive to the formation of amorphous schwertmannite in 9K medium at 10℃. Correspondingly, the generation of amorphous schwertmannite was preceded to ihleite at 28℃, and the crystallinity degree of ihleite was getting better with the extension of culture time. Combined with the SEM characteristics, it was judged that the 28℃ sample contained jarosite and schwertmannite. |
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