| 树脂D201上粗漆酶的固定化及对孔雀石绿的脱色 |
| 摘要点击 2275 全文点击 1410 投稿时间：2011-10-29 修订日期：2011-12-06 |
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| 中文关键词 粗漆酶 阴离子树脂 固定化 稳定性 脱色 |
| 英文关键词 crude laccase anion exchange resin immolization stability dye decoloration |
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| 中文摘要 |
| 云芝粗漆酶以3种方法,即静电吸附法(D201-Lac-Ⅰ)、先吸附后交联法(D201-Lac-Ⅱ)、戊二醛处理树脂后吸附法(D201-Lac-Ⅲ)固定到大孔阴离子交换树脂D201上.与静电吸附法相比,D201-Lac-Ⅱ的漆酶固载量大幅提升至4.65倍,漆酶比活力却严重受损,只有前者的4.8%; D201-Lac-Ⅲ的漆酶固载量略有下降,为静电吸附法的0.51倍,漆酶比活力却显著提高至静电吸附法的2.99倍.利用电子透射电镜(TEM)可明显观察到D201-Lac-Ⅲ漆酶分子聚合体的阴影.用D201-Lac-Ⅲ对孔雀石绿进行了连续多批次脱色试验,在长达210 h的连续操作中,其脱色效率未观察到有下降趋势,一直稳定在40%~55%之间,D201-Lac-Ⅲ的酶活亦未观察到下降,同等条件下游离的漆酶活力已损失至20%以下,证明D201-Lac-Ⅲ具有显著提升的稳定性和优良的重复利用性.考虑到粗漆酶廉价易得,D201-Lac-Ⅲ在水处理应用中可能大有前景. |
| 英文摘要 |
| Crude laccase from Trametes versicolor was immobilized onto anion exchange resin D201 by three methods, i.e., direct electrostatic adsorption (D201-Lac-Ⅰ), crosslinking after electrostatic adsorption (D201-Lac-Ⅱ) and electrostatic adsorption after treating D201 with glutaraldehyde (D201-Lac-Ⅲ). Compared to direct electrostatic adsorption, the immobilized laccase amount of D201-Lac-Ⅱ increased by 4.65 times but the laccase activity was decreased to 4.8%, while the laccase activity on D201-Lac-Ⅲ increased by 2.99 times, with the immobilization amount decreased to 51%. Shadows of laccase aggregation on D201-Lac-Ⅲ were found by transmission electron microscopy (TEM). Continuous batch decoloration of malachite green demonstrated that the decoloration efficiency of D201-Lac-Ⅲ remained in the range of 40% to 55% for more than 210 hours, in addition, the enzyme activity on D201-Lac-Ⅲ maintained unchanged while the activity of free laccase declined to less than 20% under the same condition. All of the results above indicated that D201-Lac-Ⅲ had a significantly enhanced stability and good reusability. Considering the low price and simple production procedure of crude laccase, D201-Lac-Ⅲ could be promising for water treatment purpose. |
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