| 回灌渗滤液C/N对填埋垃圾生物反应器反硝化特性的影响 |
| 摘要点击 4945 全文点击 2983 投稿时间：2011-01-05 修订日期：2011-05-05 |
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| 中文关键词 填埋垃圾生物反应器 渗滤液 C/N 反硝化特性 反硝化细菌 |
| 英文关键词 landfill bioreactor landfill leachate C/N ratio denitrification capacity denitrifying bacteria |
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| 中文摘要 |
| 利用模拟填埋垃圾生物反应器（landfill bioreactor）研究了回灌渗滤液C/N对填埋垃圾堆体反硝化性能的影响，并采用PCR扩增、克隆测序等分子生物学技术，以功能基因nirS为分子标记考察了4次回灌过程中反应器内反硝化微生物群落结构变化.结果表明，回灌渗滤液的COD/NO-3-N比例对反应器的反硝化活性具有显著性影响.当COD/NO-3-N从3.11提高到13.08时，反应器内硝酸盐还原速率可从1.14 mg·（kg·h）-1提高到11.40 mg·（kg·h）-1.当回灌渗滤液生物可利用性COD与NO-3-N比值达到6.37时，可以实现填埋垃圾生物反应器反硝化作用的快速、稳定运行.4次回灌，反应器内的反硝化微生物大部分与β-变形菌纲（β-proteobacteria）细菌相似，少数属于非培养微生物（uncultured bacteria），其中 Thiobacillus denitrificans和Azoarcus tolulyticus是反硝化过程的主要功能微生物，在回灌渗滤液反硝化过程中可能发挥着重要作用. |
| 英文摘要 |
| The denitrification capacity of a landfill bioreactor was investigated under different ratios of injected leachate C/N; the denitrifying bacteria community compositions over four injection times were also studied using molecular approaches and functional gene nirS served as marker.Results showed that COD/NO-3-N ratio of the injected landfill leachate had a significant impact on the denitrification capacity of the bioreactor.Nitrate reduction rate increased from 1.14 mg·（kg·h）-1to 11.40 mg mg·（kg·h）-1 when the injected leachate COD/NO-3-N ratio raised from 3.11 to 13.08.It suggested that a stable and rapid denitrification could be obtained when bioavailable COD/NO-3-N ratio in the injected leachate was 6.37.In the bioreactor,the main denitrifying bacteria was similar to β-proteobacteria,and others belonged to uncultured bacteria.Thiobacillus denitrificans and Azoarcus tolulyticus known as β-proteobacteria were the dominant species and played an important role in NO-3-N consumption during the leachate injection. |
