| Cd对欧美杂交杨生长紫色土和冲积土微生物多样性的影响 |
| 摘要点击 2678 全文点击 1570 投稿时间：2010-07-18 修订日期：2010-08-27 |
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| 中文关键词 镉 紫色土 冲积土 可培养微生物 微生物生物量C、N 细菌群落结构 PCR-DGGE |
| 英文关键词 Cd contamination purple soil alluvial soil culturable microorganism microbial biomass C and N bacterial community structure PCR-DGGE |
| DOI 10.13227/j.hjkx.20110743 |
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| 中文摘要 |
| 研究了现实环境中Cd污染水平对长江流域典型土壤微生物多样性的影响，分析了现实Cd浓度胁迫对盆栽欧美杂交杨(Populus deltoides×Populus nigra)生长的紫色土和冲积土中可培养的微生物数量、生物量和细菌群落多样性的影响.结果表明，Cd处理显著增加了栽植杨树的紫色土中可培养细菌和放线菌数量，降低了可培养真菌的数量和微生物生物量N含量.DGGE图谱显示细菌群落结构在不同Cd胁迫处理下也发生了明显改变.相反，栽植杨树的冲积土中可培养的细菌和真菌数量在较低浓度的Cd处理中表现出增加的趋势，而高浓度的Cd胁迫均显著降低各类可培养微生物数量及微生物生物量N含量，且不同Cd胁迫处理下的细菌群落结构差异较小.另外，施加Cd处理对冲积土和紫色土微生物生物量C含量均无显著影响.本研究结果为认识现实Cd污染水平下土壤微生物特征提供了一定的基础数据. |
| 英文摘要 |
| Effects of current Cd contamination levels on microbial biodiversity were studied under the typical Cd contaminated soils in the Yangtze Basin. Purple soil and alluvial soil potted with a poplar (Populus deltoides×Populus nigra) were selected, and the culturable soil microbial amounts by flat method, microbial biomass and bacterial community structure by PCR-DGGE were investigated. Cd supplies significantly increased the culturable amounts of bacteria and actinomyces in purple soil, but decreased the culturable amounts of fungi and the content of microbial biomass N. Fingerprint of DGGE also showed that bacterial community structure have obviously changed under different Cd supplies. In contrast, the lower Cd supplies slightly increased the culturable amounts of bacteria and fungi in alluvial soil, but higher Cd supply treatment decreased the culturable amounts of bacteria, actinomyces and fungi, and the content of microbial biomass N. However, only a slight change was observed under different Cd supplies by DGGE fingerprint. Additionally, there were few effects of Cd supplies on the content of microbial biomass C in both purple soil and alluvial soil. The results provided basic data to understand the effects of present Cd contamination levels on soil microbial characteristics. |
