| 应用T-RFLP技术分析滇池污染水体的细菌群落 |
| 摘要点击 3790 全文点击 4386 投稿时间：2010-08-05 修订日期：2010-09-20 |
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| 中文关键词 滇池 细菌群落 多样性 优势菌 T-RFLP技术 |
| 英文关键词 Dianchi Lake analysis of bacterial community diversity dominant bacteria T-RFLP |
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| 中文摘要 |
| 为确定重富营养化湖泊——滇池水体的主要细菌类型与种群结构， 本研究运用末端限制性片段长度多态性（T-RFLP）技术分析了滇池水体9个样点的细菌群落特征及多样性.对各样本的T-RFLP谱图进行聚类分析，结果表明不同地理位置的污染物浓度差异可能是造成细菌群落差异的主要原因.研究同时结合克隆文库技术对各样本T-RFLP谱图中的末端限制性片段（T-RFs）进行了分析，通过建立T-RFs长度与细菌种属的对应关系，确定这9个样点的优势菌组成主要为Pseudomonas属、Aphanizomenon属和Rhodobacter属. |
| 英文摘要 |
| In order to assess the dominant microbial communities in the polluted water of the Dianchi Lake, terminal restriction fragment length polymorphism (T-RFLP) technique was used to analyze the characteristics and diversity of bacterial communities in nine sites. Clustering analysis based on the T-RFLP profiles showed that the bacterial community structures varies considerably, which may be caused by the pollutant variation at different geographic locations. According to the further analysis of T-RFLP profiles with clone library technique, the dominant bacteria groups in nine sites represented by distinctive terminal restriction fragments (T-RFs) were identified as genera of Pseudomonas, Aphanizomenon and Rhodobacter. |
