| 2株球形棕囊藻溶藻细菌的分离及鉴定 |
| 摘要点击 2545 全文点击 2231 投稿时间：2010-03-15 修订日期：2010-04-30 |
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| 中文关键词 球形棕囊藻 溶藻细菌 溶藻特征 有害赤潮 16S rDNA 芽孢杆菌 |
| 英文关键词 Phaeocystis globosa algae-lysis bacteria algicidal characterization harmful algal bloom 16S rDNA Bacillus |
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| 中文摘要 |
| 从珠海赤潮海水中分离出2株溶藻细菌Y01和Y04，对球形棕囊藻均有显著的溶藻效果. 结果表明，溶藻细菌Y01和Y04均为革兰氏阳性菌，对球形棕囊藻的溶解作用时间为6 d.借助显微镜及扫描电镜等观察Y01和Y04溶藻过程，发现2株溶藻细菌的溶藻方式均为直接裂解藻细胞.溶藻细菌Y01和Y04的16S rDNA系列分析表明，溶藻细菌Y01和Y04的PCR扩增产物的长度分别为1468 bp和1548 bp（登录号分别为DQ531607和DQ531608）.菌株Y01和Y04与多株芽孢杆菌的16S rDNA核苷酸序列的同源性在99.7%以上，均归属于芽孢杆菌属. |
| 英文摘要 |
| The algae-lysis bacteria Y01 and Y04 were isolated from seawater, during a red tide survey of the Zhuhai Estuary in southern China. The algae-lysis bacteria Y01 and Y04 were Gram-positive bacteria which can remove P.globosa in 6 days. The lysing process was observed by microscope and SEM, both of them could directly lyse P.globosa. The strains were identified by the sequence analysis of 16S rDNA. The length of the two bacteria's sequences were 1 468 bp and 1 548 bp(strain Y01: DQ531607; strain Y04: DQ531608). The DNA sequence similarity searches showed that share more than 99.7% sequence homology with some strain of Bacillus. The algae-lysing bacteria would provide a possibility to control P.globosa red-tide by microbial agents. |
