| 络合协同RDB处理NOx系统中1株反硝化菌的分离鉴定 |
| 摘要点击 2594 全文点击 1039 投稿时间：2009-09-15 修订日期：2010-01-06 |
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| 中文关键词 生物转鼓 兼氧反硝化细菌 16S rDNA序列 Pseudomonas stutzeri 系统发育学 |
| 英文关键词 rotating drum biofilter(RDB) heterotrophic anoxic denitrifier 16S rDNA sequence Pseudomonas stutzeri phylogenetic analysis |
| DOI 10.13227/j.hjkx.20100741 |
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| 中文摘要 |
| 从处理效率稳定在85%的NOx废气络合处理系统——生物转鼓（rotating drum biofilter,RDB）中分离到1株异养型兼氧反硝化细菌，命名为菌株ND1. 理化分析表明，ND1为革兰氏染色阴性杆菌，在培养基上可形成特征性干燥、皱缩样菌落，黏附于琼脂表面，并产生黄色色素，以单极生鞭毛运动.其16S rDNA基因序列与典型的反硝化菌Pseudomonas stutzeri具有97%的相似性，综合其外部形态特征、生理生化特征、Biolog碳源利用特性以及16S rDNA系统发育学分析，ND1鉴定为Pseudomonas stutzeri.在30℃，pH 7.2的培养条件下，以琥珀酸钠为碳源，5 d后该菌对硝酸根离子的去除率可以达到100%，对相同浓度的亚硝酸根离子的去除率达到85%. |
| 英文摘要 |
| A heterotrophic denitrifying strain named ND1 has been isolated from rotating drum biofilter(RDB) which was mainly used for the treatment of exhaust gas such as NOx.It was proved to be Gram-negative.In addition,it was able to form dry and wrinkled colonies on the medium,adhere on the agar surface,produce yellow pigments and be motile with a single polar flagellum.It was indentified as the Pseudomonas stutzeri according to morphological and physiological biochemical characteristics and 16S rDNA sequence analysis.Under the conditions of pH 7.2 and temperature 30℃,the NO-3-N removal efficiency by ND1 was up to 100% in 5 days,while the NO-2-N degradation ratio was only 85% by the strain DN1 when subjected to the same condition as the above. |
