| 厌氧折流板反应器酸化及其对微生物种群分布的影响 |
| 摘要点击 6630 全文点击 3944 投稿时间：2009-09-16 修订日期：2010-02-09 |
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| 中文关键词 厌氧折流板反应器 酸化 种群演替 荧光原位杂交 |
| 英文关键词 anaerobic baffled reactor acidification population succession fluorescence in situ hybridization |
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| 中文摘要 |
| 研究了厌氧折流板反应器（ABR）酸化过程中pH、COD、挥发性脂肪酸（VFA）和微生物表观形态的变化，并基于荧光原位杂交（FISH）定量解析了酸化中厌氧微生物的种群演替过程.结果表明，酸化过程由ABR反应器前端向后端逐渐推进；完全酸化后，各隔室运行效率明显降低，COD去除率仅为30.9%，pH值下降1.0～2.2，出水VFA增长5.1倍.酸化显著影响微生物形态，导致颗粒污泥表层和内部微生物出现畸变甚至死亡，造成颗粒内外传质困难.基于FISH技术的定量研究表明，酸化促进了真细菌的繁殖，抑制了古细菌的生长，导致厌氧消化过程中3种关键菌群的大量减少，其中食丁酸盐产氢产乙酸菌Syntrophomonas spp.减少了30.9%，食丙酸盐产氢产乙酸菌Syntrophobacter wolinii 减少了85.5%，耗氢产乙酸菌E. limosum减少了60.0%，产甲烷菌Methanomicrobiales减少了54.3%，微生物种群间物质供需平衡被破坏. |
| 英文摘要 |
| The changes of pH, COD, volatile fatty acids (VFA) and microbial morphology of the acidification process in an anaerobic baffled reactor (ABR) were investigated. And the population succession process of the anaerobic microorganisms was quantitatively analyzed by using the Fluorescent In situ hybridization technology (FISH). The results show that the ABR reactor is acidified gradually from the front to the back. After the reactor is entirely acidified, the COD removal efficiency is only 30.9%, and the pH values are lowered by 1.0-2.2, while the VFA in effluent increases by 5.1 times. Additionally, the microbial morphology is significantly affected by the acidification process, in which not only the bacteria are deformed or died, but also the internal and external mass transfer of granular sludge becomes difficult. The quantitative analyses with FISH shows that in the acidification process the Archaea growth is inhibited but the Eubacteria growth is promoted, thus resulting in the sharp decrease of the three crucial microorganisms of the anaerobic digestion. The abundance of the butyrate-oxidizing acetogenic bacteria Syntrophomonas spp. reduces by 30.9%, the propionate-oxidizing acetogenic bacteria Syntrophobacter wolinii reduces by 85.5%, the homoacetogenic bacteria Acetobacterium species E. limosum reduces by 60.0%, and methanomicrobium Methanomicrobiales reduces by 54.3%. All these result in the upsetting of the mass transfer balances of different anaerobic microorganism populations. |
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