| T-RFLP方法分析城市污水处理厂中细菌群落的动态变化 |
| 摘要点击 7042 全文点击 3387 投稿时间：2009-07-06 修订日期：2009-08-31 |
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| 中文关键词 细菌 群落动态变化 T-RFLP 移动窗口分析 洛伦兹曲线 城市污水处理厂 |
| 英文关键词 bacteria community dynamics terminal restriction fragment length polymorphism (T-RFLP) moving window analysis Lorenz curve wastewater treatment plant |
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| 中文摘要 |
| 采用基于16S rRNA基因的末端限制性片段长度多态性技术(T-RFLP)，分析了功能稳定的2个城市污水处理系统中（系统A及系统B），细菌群落结构在105 d中的动态变化.在研究中，系统A和系统B功能稳定，BOD出水浓度均在8 mg/L左右，总氮（TN）出水浓度分别在25 mg/L和23 mg/L之下.T-RFLP图谱分析表明，系统A中，115、 118、 123、 471、 482和835 bp等T-RFs的相对丰度变化显著，系统B中，123、 471、 750和835 bp等T-RFs的相对丰度变化显著.移动窗口分析表明，系统A中细菌群落的平均变化率Δt(15 d) 为21.5%±5.7%，系统B的平均变化率Δt(15 d) 为22.6%±5.0%.洛伦兹曲线分析表明，系统A中20%的微生物的累积相对丰度为43%～50%；系统B中20%的微生物的累积相对丰度为47%～52%. 结果表明，在城市污水处理厂中，稳定的功能并没有藕联着稳定的细菌群落结构. |
| 英文摘要 |
| Bacterial communities in two full-scale wastewater treatment systems (system A and system B) with functional stability were monitored over 105 days using terminal restriction fragment length polymorphism (T-RFLP) based on 16S rRNA genes. During the study period, system A and system B were functionally stable. The effluent BOD concentration of two systems was always around 8 mg/L, and at the same time the effluent TN concentrations of two systems were always below 25 mg/L and 23 mg/L respectively. However, the T-RFLP profiles showed that for system A, the relative abundance of T-RF of 115, 118, 123, 471, 482 and 835 bp changed significantly. For system B, the T-RF of 123, 471, 750 and 835 bp changed significantly. The moving window analysis showed the average community change rates (every 15 days) of system A and system B were 21.5%±5.7% and 22.6%±5.0% respectively.Based on the Lorenz curves, it was observed for system A that 20% of the species corresponded with 43% to 50% of the cumulative relative abundance. For system B, 20% of the species corresponded with 47% to 52% of the cumulative relative abundance. In conclusion, the results of this study clearly revealed that, in the full-scale wastewater treatment systems, the functional stability did not correlate to the stable bacterial communities. |
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