| 典型水稻土微生物RNA的提取方法比较 |
| 摘要点击 2508 全文点击 2399 投稿时间：2009-05-19 修订日期：2009-10-14 |
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| 中文关键词 水稻土 微生物RNA提取 RT-PCR nosZ基因 |
| 英文关键词 paddy soil microorganism RNA extraction RT-PCR nosZ gene |
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| 中文摘要 |
| 采用4种方法分别提取和纯化2种典型土壤微生物RNA（水稻土和旱地土），并对RNA产量和质量进行比较评价.结果表明，方法1用液氮研磨法提取的RNA提取量最高，但需要纯化后才能满足RT-PCR等后续分子生物学要求；方法2用玻璃珠破碎法提取的RNA提取量稍低，但RNA纯度较高，可以直接用于RT-PCR等后续实验；方法3用试剂盒提取的RNA纯度高，但提取量最低，成本高，且对土壤样品有选择性；方法4未能提出供试土壤的微生物RNA.选择RNA提取效果较好的方法1和方法2并应用于3种典型水稻土（紫潮泥、红黄泥、麻沙泥），结果表明，这2种方法也适合于这3种水稻土微生物RNA的提取. |
| 英文摘要 |
| Four different methods were evaluated for extracting RNA from microorganisms of a paddy soil and a upland soil， the RNA quality and quantity were examined. The results showed that method 1 applying liquid nitrogen for grinding gave the highest yield but poor purity， and the additional purification was necessary for further RT-PCR. Method 2 using bead beating yielded the RNA with high purity which could be directly used for RT-PCR， but the RNA yield was lower than that of method 1.Method 3 with the commercial soil RNA extraction kit achieved high quality RNA but the lowest yield and had selectivity for soils. Method 4 is not suitable for extracting RNA from the test soils. Method 1 and method 2 with good performances on soil RNA extraction were applied to other three typical paddy soils including purple calcareous clayey soil， reddish yellow loamy soil， granitic sandy soil. The results showed that method 1 and method 2 were suitable for extracting high quality RNA from these samples， so they might be the effective methods of RNA extraction for paddy soils. |
