| 长期施用氮肥对水稻土亚硝酸还原酶基因多样性的影响 |
| 摘要点击 3028 全文点击 3426 投稿时间：2009-04-02 修订日期：2009-05-18 |
| 查看HTML全文
查看全文 查看/发表评论 下载PDF阅读器 |
| 中文关键词 氮肥 水稻土 反硝化 nirK nirS 多样性 |
| 英文关键词 nitrogen fertilizer paddy soil denitrification nirK nirS diversity |
| DOI 10.13227/j.hjkx.20100224 |
|
| 中文摘要 |
| 以中国科学院桃源农业生态试验站长期定位试验的土壤样品为对象，采用PCR扩增、克隆测序等分子生物学技术，研究长期施氮肥对水稻土亚硝酸还原酶基因nirK、nirS多样性的影响．序列分析结果表明，从水稻土中克隆的系列nirK基因片段与NCBI数据库中未知菌种的nirK基因相似性较高，平均达90.7%；而nirS基因片段与数据库中已知的nirS基因相似度低，平均74.7%．通过Chao1估计值预测，nirK基因在不施 |
| 英文摘要 |
| In order to investigate the effects of long-term application of nitrogen fertilizer on soil denitrifying communities,the diversities of nir genes (nirK and nirS) were studied using molecular approaches in the long-term paddy field experiment (started in 1990) located in Taoyuan. Analysis of clone sequences indicated that the nirK fragments from paddy soil showed close similarity (90.7%) to the nirK sequences registered in GenBank database,but were not related to any known strain. Whereas,most of the nirS clones showed low similarity (74.7%) to the nirS gene fragments registered in GenBank. The Chao1 estimates showed that the diversity of nirK gene communitiy appeared to be higher in CK treatment [（58±13） OTUs] than in N treatment [(49±9） OTUs],but the difference was not significant. However,application of nitrogen fertilizer resulted in significant difference of nirS-community compared to CK. Nitrogen fertilizer had obvious effect on the community structure of nirK-denitrifiers (p<0.022),but the nirS-containing community was not affected. Based on phylogenetic analysis,nirK clones grouped into three clusters with aggregations of some OTUs cloned from N treatment. Although nirS clones grouped into four clusters,the majority of the clones were attributed in one cluster. The results suggested that application of nitrogen fertilizer had a greater influence on the diversity of nirS-containing bacterial community than that of the nirK. However,the community structure of nirK-containing denitrifiers was more sensitive to nitrogen fertilization than that of the nirS. |
