| pH和铜离子对微囊藻毒素-LR荧光免疫检测的影响与消除 |
| 摘要点击 2924 全文点击 2098 投稿时间：2008-08-04 修订日期：2008-10-20 |
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| 中文关键词 微囊藻毒素-LR 铜离子 荧光免疫检测 倏逝波 光纤免疫传感器 |
| 英文关键词 microcystin-LR Cu2+ fluorescence immunoassay evanescent wave fiber-optic immunosensor |
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| 中文摘要 |
| 研究了pH和铜离子对倏逝波全光纤免疫传感器检测微囊藻毒素-LR（MC-LR）的影响及其消除措施.研究表明，过酸或过碱条件对MC-LR免疫检测均有较强烈的影响，当pH<6或pH>8时，系统检测的信号随pH的降低或增大明显下降；而当pH在6～8之间时，检测标准曲线的IC50为1.01～1.04 μg/L，检测区间在0.12～10.5 μg/L之间，较适合MC-LR的检测.低浓度铜离子对MC-LR免疫检测的影响不大，当CuSO4浓度>5 mg/Ｌ时，系统检测荧光信号明显下降，而当CuSO4浓度达到10 mg/Ｌ时，系统检测信号下降70％以上.在预反应混合物中添加1%的螯合剂EDTA，能有效抑制铜离子对免疫检测的影响. |
| 英文摘要 |
| The effects of pH and Cu2+ on the detection of the Microcystin-LR based on evanescent wave all-fiber immunosensor (EWAI) and their elimination were studied. The experimental results indicated that both low pH and high pH greatly affected the immunoassay results of MC-LR. When pH was less than 6 or higher than 8, the fluorescence signals detected by immunosensor system obviously reduced with the decrease or increase of pH. While pH ranged from 6 to 8, IC50 and the linear working range of MC-LR calculated from the calibration curve were 1.01-1.04 μg/L and 0.12-10.5 μg/L, respectively, which was favourable for MC-LR immunoassay. Low concentration Cu2+ rarely affected the detection performance of MC-LR. When the concentration of CuSO4 was higher than 5 mg/Ｌ, the fluorescence signal detected by EWAI clearly decreased. Furthermore, when the concentration of CuSO4equalled to 10 mg/Ｌ, the fluorescence signal detected reduced 70%. The influence of Cu2+ on the immunoassay could effectively be compromised when 1% EDTA was added to the pre-reaction mixture. |
