| 1株类球红细菌及其降解敌敌畏的特性 |
| 摘要点击 4815 全文点击 2670 投稿时间：2008-06-24 修订日期：2008-11-30 |
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| 中文关键词 类球红细菌 敌敌畏 降解 16S rDNA 白菜 |
| 英文关键词 Rhodobacter sphaeroides dichlorvos degradation 16S rDNA Chinese cabbage |
| DOI 10.13227/j.hjkx.20090443 |
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| 中文摘要 |
| 类球红细菌具有广泛的代谢方式，它能够发酵生产5-氨基乙酰丙酸、辅酶Q10、类胡萝卜素、氢气等，已经成为一种非常有工业化开发潜力的微生物.从土壤中分离、筛选了１株类球红细菌（编号为EBL0706），分析了它的培养和遗传特性，测定了水溶液的pH、温度、敌敌畏浓度、类球红细菌浓度对其降解敌敌畏速率的影响，结果表明，该菌株在水溶液中能够快速矿化敌敌畏，在pH 6.9～7.5、温度20～50℃条件下，在5×108 CFU/mL的类球红细菌稀释液中，400 mg/L的敌敌畏在12 h内降解率达到98％以上.该菌株发酵液的稀释液喷洒到幼苗期的白菜叶片上，能够显著加快残留敌敌畏降解速度，显示出其在无公害农产品生产中的应用潜力. |
| 英文摘要 |
| Rhodobacter sphaeroides possesses an extensive range of energy acquiring mechanisms including photosynthesis, lithotrophy, aerobic and anaerobic respiration. It can produce 5-aminolevulinic acid, CoQ10, carotenoids, hydrogen, etc． by fermentation. A Rhodobacter sphaeroides strain was isolated, designated as EBL0706, from soil. The degradation of dichlorvos (DDVP) by the Rhodobacter sphaeroides was investigated. 98% of DDVP could be degraded in water solution in 12 h when 5×108 CFU/mL Rhodobacter sphaeroides was added to 400 mg/L DDVP solution under pH 6.9-7.5 and 20-50℃. This strain could also degrade the DDVP residues on Chinese cabbage leaves effectively. |
