| 不动杆菌（Acinetobacter sp.）降解4-氯酚的特性及机制研究 |
| 摘要点击 2741 全文点击 1687 投稿时间：2007-11-01 修订日期：2007-12-14 |
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| 中文关键词 氯酚 生物降解 优先污染物 酶活性 裂解途径 |
| 英文关键词 chlorophenol biodegradation priority pollutant enzymatic activity cleavage pathway |
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| 中文摘要 |
| 富集培养从受污染土壤中分离到的能够以4-氯酚为唯一碳源和能源的微生物，16S rDNA序列分析表明，该微生物为Acinetobacter sp..其降解4-氯酚的机制为邻位裂解途径，氯代邻苯二酚1,2-双加氧酶的活性可以通过氯酚的诱导显著提高.当氯酚的初始浓度范围为2～8 mmol/L时，该微生物能够很好地生长，并能有效地降解氯酚.除4-氯酚外，该微生物还可以降解2-氯酚、3-氯酚和2,4-二氯酚，有较宽的底物范围.添加柠檬酸等共基质不仅能够改善微生物的生长，还可以提高氯酚的降解效率，这对于实际受污染环境的生物修复非常重要. |
| 英文摘要 |
| The bacterium capable of degrading 4-chlorophenol (4-CP) was enriched and isolated from agricultural soil.It was gram-negative，rod-shaped bacterium，and identified as Acinetobacter sp.based on the analysis of the 16S rRNA gene fragment.It was able to utilize 4-CP as a sole carbon source.The degradation mechanism of 4-CP by this isolate was proposed as a modified ortho-cleavage pathway，the activity of chlorocatechol 1，2-dioxygenase was markedly induced.The bacterial isolate grew well and exhibited a high degradation efficiency when the initial concentration of 4-CP was between 2-8 mmol/L.It was able to survive in the presence of 4-CP at higher concentrations (up to 8 mmol/L).Not only 4-CP，but also 2-chlorophenol，3-chlorophenol，phenol，and 2，4-dichlorophenol，were also growth substrates for the isolate.The results of co-substrate supplementation illustrated the suitable conditions of the isolate to improve growth rate and 4-chlorophenol biodegradation efficiency.The results suggested that the isolate had a potential use for bioremediation of the site contaminated with 4-chlorophenol. |
