| Mn2+在黄杆菌FCN2菌株降解芘过程中的作用研究 |
| 摘要点击 2630 全文点击 1505 投稿时间：2007-09-04 修订日期：2007-11-05 |
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| 中文关键词 黄杆菌属 芘 生物降解 Mn2+ |
| 英文关键词 Flavobacterium sp. pyrene biodegradation Mn2+ |
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| 中文摘要 |
| 利用紫外分光光度法和原子吸收分光光度法研究了Mn2+在菌株FCN2生长细胞、悬浮细胞、粗酶液降解芘时的影响作用.在菌体生长期加入0.1～5 mmol/L的Mn2+，对菌体的生长及生长量无影响，但对芘的降解均有促进作用，以加入0.1mmol/L的Mn2+效果最好，其对芘的平均降解率是对照的1.26倍.此时菌株富集的Mn2+为0.025mmol/L；在用无外加Mn2+培养的菌株FCN2悬浮细胞降解芘时，加入0.5 mmol/L的Mn2+，芘的平均降解率为对照的1.67倍.降解反应发生72h后菌株富集的Mn2+为0.18mmol/L；在酶促降解时加入0.1mmol/LMn2+，平均降解率为对照的1.30倍.结果表明，在不同时期加入的Mn2+对降解芘均有一定的促进作用. |
| 英文摘要 |
| Mn2+ concentration affecting polycyclic aromatic hydrocarbon pyrene degradation by the Flavobacterium sp.FCN2 was investigated by adding Mn2+ during several periods,which including bacteria cultivation period,bacteria degrading pyrene and enzymatic degradation period.Results show the concentration of Mn2+ was beneficial for pyrene degradation,and Mn2+ was no influential to bacteria growth and magnitude.When Mn2+ was added during culturing strain FCN2 period,bacteria FCN2 can enrich Mn2+,and pyrene removal efficiency by Mn2+ enrichment bacteria is 1.26 times more than that of without Mn2+ enrichment bacteria.As well as the situation that adding Mn2+ during FCN2 degrading pyrene period that pyrene removal efficiency is 1.67 times more than that of without Mn2+.And when adding Mn2+ during enzymatic degradation periods,the elimination efficiency of pyrene was 1.30 times more than that of without Mn2+. |
