首页  |  本刊简介  |  编委会  |  投稿须知  |  订阅与联系  |  微信  |  出版道德声明  |  Ei收录本刊数据  |  封面
环境水体中肠道病原细菌的定量PCR检测
摘要点击 3943  全文点击 2371  投稿时间:2007-05-21  修订日期:2007-10-15
查看HTML全文 查看全文  查看/发表评论  下载PDF阅读器
中文关键词  地表水  通用引物  病原细菌  定量PCR  膜过滤
英文关键词  surface water  universal primer  enteric pathogenic bacteria  quantitative polymerase chain reaction(QPCR)  membrane filtration (MF)
DOI    10.13227/j.hjkx.20080504
作者单位
刘永军 西安建筑科技大学西北水资源生态与环境教育部重点实验室西安 710055 
张崇淼 西安建筑科技大学西北水资源生态与环境教育部重点实验室西安 710055 
王晓昌 西安建筑科技大学西北水资源生态与环境教育部重点实验室西安 710055 
吕英俊 西安建筑科技大学西北水资源生态与环境教育部重点实验室西安 710055 
左丽丽 西安建筑科技大学西北水资源生态与环境教育部重点实验室西安 710055 
中文摘要
      采用通用引物,通过实时荧光定量PCR方法(QPCR),对西安市5处地表水体中肠道病原细菌的细胞密度进行4个月的连续检测,并将QPCR检测结果与滤膜法测得的大肠菌群CFU值进行比较分析.结果显示, QPCR法可信度为94%,最小检测值为每管未稀释DNA提取物含2.7个大肠杆菌细胞.5个水体(N=60)检测结果表明,QPCR检测结果是大肠菌群CFU的2.2~5倍.病原细菌的几何平均值范围,QPCR法在25~67?000 CCE/100 mL之间,MF法大肠菌群为3~45?000 CFU/100 mL之间.2种方法的离散和回归分析表明,QPCR检测结果与大肠菌群CFU显著正相关,秩相关系数为R=0.983.
英文摘要
      A rapid quantitative polymerase chain reaction (QPCR) analysis method with universal primers was developed to detect cell densities of the enteric pathogenic bacteria from 5 surface water of Xi'an City for 4 months continuously. And the detection results by QPCR method were compared with counts of coliforms colony-forming units (CFU) determined by membrane filter (MF) analysis. The results showed that QPCR method had an estimated 94% confidence, and detection limit was 2.7 Escherichia coli cells per sample in undiluted DNA extracts. For five surface waters(N=60), the geometric mean of pathogenic bacteria concentration determined by QPCR was 2.2-5 times of corresponding coliform CFU determined by MF analysis. Using QPCR analysis, these geometric means of pathogenic bacteria concentration ranged from 25 CCE/100 mL to 67?000 CCE/100 mL. Using MF culture analysis, coliforms ranged from 3 CFU/100 mL to 45?000 CFU/100 mL. Regression analysis showed that there was a significant positive correlation between pathogenic bacteria determined by QPCR method and coliforms determined by MF method, the correlation coefficient (R) was 0.983.

您是第83078283位访客
主办单位:中国科学院生态环境研究中心 单位地址:北京市海淀区双清路18号
电话:010-62941102 邮编:100085 E-mail: hjkx@rcees.ac.cn
本系统由北京勤云科技发展有限公司设计  京ICP备05002858号-2