| 人工纳米材料富勒烯（C60）低剂量长期暴露对鲫鱼的氧化伤害 |
| 摘要点击 7914 全文点击 2745 投稿时间：2007-05-02 修订日期：2007-07-24 |
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| 中文关键词 纳米材料 富勒烯 鲫鱼 暴露 生态毒性 氧化伤害 |
| 英文关键词 nanomaterials fullerene C60 Carassius auratus exposure ecotoxicity oxidative damages |
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| 中文摘要 |
| 为更好地判断人工纳米材料（富勒烯，C60）对水生生物的潜在健康危害，以鲫鱼（Carassius auratus）幼鱼为受试生物，研究了低剂量C60（0.04～1.0 mg·L-1）长期（32 d）暴露对鲫鱼的氧化伤害.结果表明，各实验组中鲫鱼幼鱼脑、肝脏、鳃组织中的还原型谷胱甘肽（GSH）含量都发生显著降低 (p＜0.05)，其中1 mg·L-1 nC60/aq的暴露对鳃组织GSH含量的抑制率为14.3%，高于对鱼脑、肝脏组织中的抑制率；肝脏组织中过氧化氢酶（CAT）和超氧化物歧化酶（SOD）活性，以及鳃组织中Na+-K+-ATP酶活性均被显著激活，其最大活性分别是对照的121.34%（0.04 mg·L-1暴露组）、114.80%（0.04 mg·L-1暴露组）和348.59%（0.20 mg·L-1暴露组）.实验结果揭示，长期暴露引起机体组织的氧化应激可能是水环境中C60的致毒机制之一. |
| 英文摘要 |
| To determine the potential effects of fullerenes (C60) on aquatic organism, larval Carassius auratus was exposed to low level C60 (0.04-1.0 mg·L-1) for 32 days. Then the oxidative damages in brain, liver and gill tissue of larval Carassius auratus were measured. The results showed that: compared to the control, the reduced glutathione (GSH) contents in all different tissues decreased significantly (p＜0.05), and the most serious inhibition of GSH with a 14.3% inhibition rate was found in the gill tissue of larval crucian exposed to 1 mg·L-1 C60 aqueous suspension; whereas the superoxide dismutase (SOD) and catalase (CAT) activities in liver tissue, Na+-K+-ATPase activities in gill tissue were stimulated significantly (p＜0.05), and the maximal activity of them were 121.34% (exposed to 0.04 mg·L-1 C60 aqueous suspension), 114.80% (exposed to 0.04 mg·L-1 C60 aqueous suspension) and 348.59% (exposed to 0.20 mg·L-1 C60 aqueous suspension) respectively than that of control. The experiment results indicated that oxidative damages induced by long\|term exposure might play a role in the bio-toxicity of C60 to larval crucian. |
