| 四氧化三铁明胶固定过氧化物酶催化降解五氯酚 |
| 摘要点击 2657 全文点击 1515 投稿时间：2007-01-19 修订日期：2007-04-06 |
| 查看HTML全文
查看全文 查看/发表评论 下载PDF阅读器 |
| 中文关键词 催化去除 PCP HRP 固定化 pH 缓冲体系 |
| 英文关键词 catalytic removal PCP HRP immobilization pH buffer system |
|
| 中文摘要 |
| 利用Fe3Ｏ4吸附-明胶包埋-交联法固定辣根过氧化物酶（HRP）催化去除水中的五氯酚（PCP），探讨了催化反应时间、不同缓冲体系及pH值、不同PCP初始浓度和HRP使用量对催化反应的影响，确定了固定化HRP催化去除PCP的最佳反应条件，并与自由酶的催化反应做对比研究.结果表明，用固定化辣根过氧化物酶催化去除五氯酚，反应在30 min后可达到平衡，催化速度和自由酶相当；pH值介于 4～6之间有较明显的去除效果，固定后催化反应的适宜pH范围更为宽泛，且受不同缓冲体系影响较小，pH为5时有最高催化去除率41%；在0.05 U/mL的低活性浓度下固定化HRP的催化效率高，与自由酶相比可减少酶用量；PCP的去除量随其初始浓度的增加而增加，但催化去除率从39.68%下降至24.4%；此外，固定化HRP具有良好的催化稳定性，在酶活性浓度为0.05 U/mL时重复使用7次后PCP的催化去除率仍高于39%. |
| 英文摘要 |
| The removal of PCP using HRP immobilized by Fe3Ｏ4 sorption-gelatin embedding-cross linkage method as catalyzer was studied. Reaction conditions including the reacting time, different buffer systems and pH value, PCP initial concentration, HRP dosage were discussed in detail compared with free HRP. The results indicate that the equilibrium time of PCP removal reaction catalyzed by immobilized HRP is about 30 min, which is as fast as free HRP. The optimal pH for PCP removal by immobilized HRP is between 4～6, the proper pH should be more extensive than using free HRP. The highest catalyzing removal percent is 41% at pH 5. Low concentration of immobilized HRP can remove PCP effectively. PCP removal quantity increases but the removal percent decreases with the increase of initial concentration of PCP. The catalyzing removal percent goes down from 39.68% to 24.4%. Immobilized HRP can remove PCP repetitively. The catalyzing removal percent is still above 39% at 0.05 U/mL dosage of HRP when using 7 times repeatedly. |
