| 1株低温石油烃降解菌的分类鉴定及降解特性研究 |
| 摘要点击 3032 全文点击 2558 投稿时间：2006-11-15 修订日期：2007-01-09 |
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| 中文关键词 低温 烃降解 红球菌 生物修复 |
| 英文关键词 low temperature hydrocarbons-degrading Rhodococcus sp. bioremediation |
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| 中文摘要 |
| 从渤海油船泄漏区域的海底泥中筛选到1株能降解柴油的低温石油烃降解菌T7-2，初步鉴定为红平红球菌(Rhodococcus erythropolis).研究表明，利用工业乙醇无机盐培养基培养液体种子是适宜的，其最适温度和pH分别为15℃和7.8，工业乙醇最适加入量为0.5％，菌体浓度为108 CFU/mL.接种人工海水烃降解培养基后，通过补加氮源 (NH4)2SO4 2.64 g/L、磷源Na2HPO4 2.5 g/L和酵母粉 0.015 g/L后，15℃振荡培养7 d，降解率可以达到73.2％.该菌降解烷烃的范围很广泛，C12～C36均有不同程度的降解. |
| 英文摘要 |
| A low-temperature hydrocarbon-degrading strain T7-2 was isolated from sea-mud of Bohai polluted area and identified as Rhodococcus erythropolis, which could use diesel oil as carbon source. The optimal temperature and pH for the strain utilizing ethanol was 15℃ and 7.8, and the optimal concentration of ethanol and the seed culture was 0.5% and 108 CFU/mL, respectively. Inoculated to artificial seawater which was added (NH4)2SO4 2.64 g/L、Na2HPO4 2.5 g/L and yeast extract 0.015 g/L after 7 days of culture at the temperature of 15℃, the rate of degradation was 73.2％.The strain could degrade a large range of n-alkane from C12 to C36. |
