| 微囊藻毒素［Dha7］MCRR的制备及鉴定 |
| 摘要点击 3602 全文点击 3046 投稿时间：2006-11-07 修订日期：2006-12-23 |
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| 中文关键词 ［Dha7］MCRR 纯化 鉴定 |
| 英文关键词 ［Dha7］microcystin RR purification identification |
| DOI 10.13227/j.hjkx.20070930 |
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| 中文摘要 |
| 以野外收集的水华蓝藻为原料，经过75％甲醇溶液浸提，快速色谱分离和半制备色谱纯化，从滇池水华蓝藻中分离纯化出1种微囊藻毒素变体.电喷雾质谱、紫外分光光度计和HPLC检测结果表明，所得毒素为［Dha7］MCRR，是MCRR的1种去甲基化变体，其纯度大于 95％.该毒素的分子组成为环(Ala-Arg-MeAsp-Arg-Adda-Glu-Dha)，分子量为1 023，其紫外扫描光谱（200～300 nm）在239 nm处有特征吸收.［Dha7］MCRR在滇池水华蓝藻中普遍存在，有时会成为MC的主要种类. |
| 英文摘要 |
| One variant of microcystins was isolated and purified with cyanobacteria natural bloom as the starting material, which was collected in Dianchi Lake, China. The separation protocol involved extraction of cyanobacterial cells by 75% aqueous methanol, isolation by reversed-phase flash chromatography, and purification by reversed-phase semipreparative HPLC. The structure and purity of purified microcystin was identified with electrospray ionization mass spectrometry, UV spectrophotometer, and analytical HPLC. The purified microcystin was assigned as ［Dha7］MCRR (purity >95%), which was a demethylated variant of MCRR. The structure of purified microcystin was identified as cyclo-(Ala-Arg-MeAsp-Arg-Adda-Glu-Dha) with molecular weight of 1 023. There was a maximum absorbance at 239 nm in its UV spectrum (200～300 nm). This variant of microcystins occurred frequently, and sometimes could become the main variant in waterbloom from Dianchi Lake. |
