| 降解1,2,4-三氯苯的硝基还原假单胞菌J5-1的分离鉴定和邻苯二酚1,2-双加氧酶基因的克隆 |
| 摘要点击 3748 全文点击 2304 投稿时间：2006-09-08 修订日期：2006-10-26 |
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| 中文关键词 硝基还原假单胞菌J5-1 1,2,4-三氯苯 生物降解 降解动力学 氯代邻苯二酚1,2-双加氧酶 |
| 英文关键词 Pseudomonas nitroreducens strain J5\|1 1,2,4-trichlorobenzene biodegradation degradation dynamics chlorocatechol 1,2-dioxygenase |
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| 中文摘要 |
| 从受氯苯污染的土壤中分离到1株以1,2,4-三氯苯为唯一碳源生长的细菌,命名为J5-1.根据其生理生化特征和16S rDNA(GenBank Accession No. EF107515)序列相似性分析,将该菌株初步鉴定为硝基还原假单胞菌(Pseudomonas nitroreducens).当1,2,4-三氯苯初始浓度为400 mg/L时, J5-1对其最大降解率接近90％;当1,2,4-三氯苯浓度初始为20 mg/L时,降解效果最好.J5-1对1,2,4-TCB的降解服从一级反应动力学.从J5-1的基因组DNA中克隆到CC12O的全长序列. |
| 英文摘要 |
| A bacterium capable of utilizing 1,2,4-trichlorobenzene as sole carbon source was isolated from the polluted soil sample. This baterium was identified as Pseudomonas nitroreducens according to its physiological & biochemical analysis and its 16S rDNA sequence(GenBank Accession No. EF107515). When the initial concentration of 1,2,4-TCB is 400 mg/L, J5-1 can achieve a maximum degradation rate of 90%. When the initial concentration of 1,2,4-TCB is 20 mg/L, the effect of degradation is the best. Degradation of 1,2,4-TCB by strain J5-1 obeys the first order dynamics. The total gene of chlorocatechol 1,2-dioxygenase was cloned from genomic DNA of J5-1. |
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