| 1,2,4-三氯苯矿化菌的鉴定与功能分析 |
| 摘要点击 2482 全文点击 1872 投稿时间：2006-07-20 修订日期：2006-11-08 |
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| 中文关键词 同位素示踪法 三氯苯 生物降解 博德特氏菌属(Bordetella sp.) 16S rDNA 荧光原位杂交(FISH) |
| 英文关键词 14C-labeled TCB biodegradation Bordetella sp. 16S rDNA fluorescence in situ hybridization (FISH) |
| DOI 10.13227/j.hjkx.20070527 |
| 作者 | 单位 | | 王芳 | 中国科学院南京土壤研究所土壤与农业可持续发展国家重点实验室，南京210008 | | U.Drfler | Institute of Soil Ecology, GSF-National Research Center for Environment and Health, 85764 Neuherberg, Germany | | M.Schmid | Institute of Soil Ecology, GSF-National Research Center for Environment and Health, 85764 Neuherberg, Germany | | S.Grundmann | Institute of Soil Ecology, GSF-National Research Center for Environment and Health, 85764 Neuherberg, Germany | | J.C.Munch | Institute of Soil Ecology, GSF-National Research Center for Environment and Health, 85764 Neuherberg, Germany | | 蒋新 | 中国科学院南京土壤研究所土壤与农业可持续发展国家重点实验室，南京210008 | | R.Schroll | Institute of Soil Ecology, GSF-National Research Center for Environment and Health, 85764 Neuherberg, Germany |
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| 中文摘要 |
| 采用同位素示踪法从氯苯污染土壤中筛选到能高效降解1,2,4-三氯苯(1,2,4-TCB)为CO2的细菌E3和F2，根据其表型特征、16S rDNA序列和荧光原位杂交(FISH)分析，鉴定E3和F2为博德特氏菌（Bordetella sp.）.E3和F2的16S rDNA序列相似性为100%；它们与菌株Bordetella sp. QJ2-5同源性最高，与Bordetella petrii(GDH030510)最接近，同源性分别为100%和99.4%.E3和F2能在含1,2,4-TCB的无机盐培养基中彻底降解1,2,4-TCB生成CO2，30 d降解率达到90%以上，其中58%和46%矿化为CO2，同时形成一定量的微生物生物量. |
| 英文摘要 |
| Two strains, E3 and F2, capable to mineralize 1,2,4-trichlorobenzene(1,2,4-TCB) were isolated from a chlorinated benzenes contaminated soil using 14C-1,2,4-TCB as carbon source. They were identified by their 16S rDNA coding genes and fluoresence in situ hybridization (FISH) analysis as members of the genus Bordetella. A similarity of 100% were observed between strains E3 and F2 with their 16S rDNA sequences. They had the highest homology of 100% with Bordetella sp. QJ2-5 and the closest relation to described species, Bordetella petrii (GDH030510) with a similary of 99.4%. Strains E3 and F2 could degrade about 90% of 1,2,4-TCB and mineralize 58% and 46% of 1,2,4-TCB to CO2 within 30 days in mineral liquid cultures, respectively. Biomass was formed during the mineralization process. |
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