| 自然土壤中苯酚降解菌的分离和系统发育分析 |
| 摘要点击 2960 全文点击 1347 投稿时间：2006-04-29 修订日期：2006-06-23 |
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| 中文关键词 自然土壤 苯酚降解菌 系统发育分析 |
| 英文关键词 natural soil phenol-degrading microorganisms phylogenetic analysis |
| DOI 10.13227/j.hjkx.20070332 |
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| 中文摘要 |
| 采用富集培养的方法，从未受苯酚污染的自然土壤中分离得到5株能以苯酚为唯一碳源生长的细菌.根据生理生化特性和系统发育分析对它们进行了初步鉴定,菌株PHD-2为Ralstonia sp.,菌株 PHD-4为Acinetobacter sp.,菌株PHD-5 为Microbacterium sp.,菌株PHD-1和PHD-3均为Pseudomonas sp.,16S rRNA基因序列的同源性比较和系统发育分析表明自然土壤中的苯酚降解菌具有丰富的多样性. |
| 英文摘要 |
| Five bacterial strains capable of utilizing phenol as sole carbon source for growth were isolated from non-contaminated natural soil sample after enrichment in the presence of phenol. They were preliminarily identified according to their phylogenetic analysis and physiological and biochemical characteristics. Strain PHD-2, PHD-4 and PHD-5 belonged to the genera of Ralstonia, Acinetobacter and Microbacterium respectively; strain PHD-1 and PHD-3 were from the genus of Pseudomonas. Homology comparing of their 16S rRNA gene sequences and phylogenetic analysis displayed the high biodiversity of phenol-degrading microorganisms in the natural soil. |
