| 苯并噻吩脱硫菌株的筛选及脱硫活性研究 |
| 摘要点击 3150 全文点击 1484 投稿时间：2005-06-18 修订日期：2005-09-06 |
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| 中文关键词 苯并噻吩 微生物脱硫 戈登氏菌 定量分析 |
| 英文关键词 benzothiophene biodesulfurization Gordona sp. quantitative detection |
| DOI 10.13227/j.hjkx.20060628 |
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| 中文摘要 |
| 从孤岛油田油浸土样中筛选到1株能降解苯并噻吩(BT)的脱硫菌,经初步鉴定该菌为戈登氏菌属(Gordona sp.).实验证明:该菌能以类似于4S途径脱除BT及其衍生物中的硫,但是不能脱除二苯并噻吩(DBT)及其衍生物中的硫.GC-MS分析表明该途径的终产物为邻羟基苯乙醛或其异构体苯并呋喃.在以BT为唯一硫源的培养基中30℃培养48h,Gordona sp.C-6能降解0.15mmol/L的BT,终产物占发酵培养基中BT加入量的50%,其余BT在有氧培养过程中挥发.通过Matlab拟合曲线确定以邻羟基苯乙酸为标准品进行产物定量检测的方法. |
| 英文摘要 |
| A benzothiophene-desulfurizing strain was isolated and identified as Gordona sp.C-6,which could degrade benzothiophene(BT) in a way analogous to the 4S pathway.It was found strain C-6 had the ability to grow in KT-BT medium whose sole source of sulfur was BT or its derivatives,whereas it couldn't grow well in a medium with DBT and its derivatives as the sole source of sulfur,the desulfurized product was identified as 2-(2′-hydroxyphenyl) ethan-1-al or its isomer benzofuran by GC-MS analysis.Desulfurization activity of strain C6 was investigated.About 0.15mmol/L(50%) BT was desulfurized by strain C-6 after it was incubated in KT-BT medium at 30℃ for 48h,the residual BT has volatilized during aerobic fermentation.The quantitative detection of the benzothiophene-desulfurizing products was established with o-hydroxyphenylacetic acid as standard compound,the standard curve was fitted by Matlab. |
