| 微囊藻毒素-LR多克隆抗体的制备 |
| 摘要点击 2849 全文点击 1352 投稿时间：2005-03-25 修订日期：2005-06-20 |
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| 中文关键词 微囊藻毒素-LR 多克隆抗体 间接竞争ELISA |
| 英文关键词 Microcystin-LR polyclonal antibody indirect competitive ELISA |
| DOI 10.13227/j.hjkx.20060435 |
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| 中文摘要 |
| 通过对新西兰大白兔免疫自制的微囊藻毒素-LR(Microcystin-LR,MC-LR)完全抗原BSA-MC-LR,获得了质量较好的抗MC-LR的多克隆抗体,间接ELISA表明抗体的效价能达到1.5×105;固定包被抗原OVA-MC-LR,采用间接竞争ELISA测定水体中的微囊藻毒素,标准曲线表明对水样中MC-LR的检测下限为10ng/L,线性区间为30ng/L·3μg/L,能满足对饮用水和地表水中MC-LR的检测要求. |
| 英文摘要 |
| Good quality polyclonal antibody against Microcystin-LR(MC-LR) was obtained from the New Zealand rabbit immunized with the homemade complete antigen BSA-MC-LR.Indirect ELISA shows the titer of this antibody is more than 1.5×105; Indirect competitive ELISA immobilized the coating antigen OVA-MC-LR was adapted to detect MCs,the calibration curve shows that the detection lower limit is 10ng/L,and the range of quantitative detection was between 30ng/L to 3μg/L,with a good linearity,which will well potentially suit for sensitive analysis for MC-LR in drinking as well as surface water samples. |
