| 海水养殖场底泥中转化硫和磷化合物的微生物及其多样性 |
| 摘要点击 2019 全文点击 2287 投稿时间：2004-03-31 修订日期：2004-07-29 |
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| 中文关键词 海水养虾场 H2S 芽孢杆菌 硫元素代谢 磷元素代谢 |
| 英文关键词 shrimp-culturing pond hydrogen sulphide Bacillus sulfur metabolism phosphorous metabolism |
| DOI 10.13227/j.hjkx.20050232 |
| 作者 | 单位 | | 王亚南 | 中国科学院微生物研究所,北京,100080 | | 王保军 | 中国科学院微生物研究所,北京,100080 | | 戴欣 | 中国科学院微生物研究所,北京,100080 | | 焦念志 | 厦门大学海洋环境科学系，厦门 361005 | | 彭志英 | 华南理工大学食品与生物工程学院,广州,510640 | | 刘双江 | 中国科学院微生物研究所,北京,100080 |
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| 中文摘要 |
| 对福建某近海养虾场底泥环境中硫和磷2种元素的微生物代谢进行了研究.结果表明,细菌代谢有机硫和无机硫产H2S是养殖过程中造成H2S污染的主要因素,利用半胱氨酸和硫代硫酸钠产生硫化氢的细菌数量分别为 1.6×106和4.35×103 个·g-1底泥;进一步研究发现,芽孢杆菌属、盐芽孢杆菌属和微杆菌属等细菌是产H2S的优势菌群,而硫酸盐还原菌的数量较少,仅为25个·g-1,其产H2S的作用不明显.研究还发现,转化有机磷和无机磷酸盐的优势菌群属于好氧细菌,其中分解卵磷脂的细菌和产磷酸酯酶细菌的数量分别为2.17×105和 1.21×106个·g-1,转化磷酸钙的细菌数量为6.96×103个·g-1.本文从微生物学的角度探讨了养殖环境中硫、磷化合物的转化,提出细菌好氧代谢产H2S是养殖环境潜在的污染因素,给出了一些改善和修复养殖环境生态的建议. |
| 英文摘要 |
| The H2S-producing bacteria and the phosphorous-cycling bacteria in sediments from near-shore marine-cultural region were investigated. Results indicate that the bacterial H2S production in aerobic condition is the dominating process to produce H2S in the sediment of cultural pond. The total counts of H2S-producing bacteria utilizing cysteine and Na2S2O3 were 1.6×106 and 4.35×103 cells g-1 respectively. The counts of sulfate-reducing bacteria in the sediments were very little, only 2.5×101 g-1. Further results show that the bacterial counts of decomposing lecithin and secreting phosphatase were 2.17×105 and 1.21×106 g-1 respectively, bacterial counts of dissolving Ca3PO4 were 6.96×103 g-1. Traditional taxonomy and partial 16S rRNA gene sequencing on the H2S-producing and phosphate-cycling bacteria indicate that most isolates could be classified as members of the following Genera: Bacillus, Halobacillus, Microbacterium, etc. |
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