| 芦苇抑藻化感物质的分离及其抑制蛋白核小球藻效果研究 |
| 摘要点击 2502 全文点击 3366 投稿时间：2003-12-01 修订日期：2004-03-24 |
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| 中文关键词 芦苇 蛋白核小球藻 抑制 化感作用 |
| 英文关键词 Chlorella pyrenoidosa Phragmites communis Tris. inhibition allelopathy |
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| 中文摘要 |
| 采用乙醇提取、溶剂萃取法从芦苇中分离出2组能抑制蛋白核小球藻生长的化感物质. 该2组化感物质对蛋白核小球藻的半效应浓度(EC50)分别为0.68mg/L和2.65mg/L. 将前者用碱性氧化铝层析柱分离得到一组抑藻活性更强的化感物质和一组能够促进蛋白核小球藻生长的化感物质: 前者对蛋白核小球藻的EC50为0.50mg/L, 是目前报道的抑藻能力最强的化感物质; 后者在浓度为0.63mg/L时能显著促进蛋白核小球藻的生长. 芦苇化感物质可以用来控制淡水中有害藻类. |
| 英文摘要 |
| Two fractions of secondary metabolites which inhibited the growth of green alga Chlorella pyrenoidosa, was isolated by several separation techniques from the ethanol extract of the macrophyte, Phragmites communis Tris. The EC50 of the two fractions on C. pyrenoidosa were 0.68mg/L(the fraction of acidic solvent) and 2.65mg/L(the fraction of alkaline solvent), respectively. Further purification of the allelochemicals in the acidic solvent by alkaline aluminum oxide column chromatography revealed that this fraction containing inhibiting allelochemicals and stimulating allelochemicals The inhibiting allelochemicals showed more inhibitory effect and the EC50 was 0.50mg/L. The result suggests that allelochemicals in P. communis can be used as an algae inhibitor. |
