Fe和Fe2+对混合细菌产氢发酵的影响 |
摘要点击 2442 全文点击 2001 投稿时间:2003-11-18 修订日期:2004-02-12 |
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中文关键词 生物制氢 乙醇型发酵 Fe Fe2+ 产氢能力 |
英文关键词 Hydrogen bio-production ethanol-type fermentation Fe Fe2+ hydrogen production capacity |
DOI 10.13227/j.hjkx.20040410 |
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中文摘要 |
在研究Fe粉剂量和Fe2+浓度对混合细菌产氢发酵的影响基础上,确定Fe和Fe2+促进混合细菌产氢能力的最佳阈值,并对乙醇型发酵菌群在不同Fe粉和Fe2+浓度下的产氢量和最大比产氢速率进行考察和对比.结果表明,Fe粉和Fe2+对乙醇型发酵菌群的产氢能力均有明显的促进作用.以葡萄糖为底物,投加Fe2+试验中,Fe2+浓度200mg/L获得最大产氢量143.7mL/g,较对照组提高32%;Fe2+浓度50mg/L获得单位VSS最大比产氢速率21.2 mL/(h·g),较对照组提高33%.投加单质Fe试验中,Fe粉剂量1000mg/L获得最大产氢量156.1mL/g,较对照组提高44%;Fe粉剂量500mg/L获得单位VSS最大比产氢速率23.5mL/(h·g),较对照组分别提高47%.单质Fe浓度高于50mg/L时,对发酵菌群产氢的促进作用要优于同浓度下的Fe2+.同时对混合细菌中铁的全量和形态分布进行了考察.
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英文摘要 |
The effects of Fe and Fe2+ concentration in the external environment on hydrogen production capacity of ethanol type fermentative microorganism flora were investigated. The optimum range of Fe and Fe2+ concentration enhanced hydrogen production were obtained and the characteristic of fermentation types at different concentration of Fe and Fe2+ were discussed. It was demonstrated that both Fe and Fe2+ were able to enhance the hydrogen production capacity of microorganism flora. The maximum hydrogen production yield of 143.7mL·g-1 glucose and the maximum specific hydrogen production rate 21.2mL·(h·g)-1 were obtained at Fe2+ concentration of 200mg·L-1 and 50mg·L-1 . The maximum hydrogen production yield of 156 1mL·g-1 glucose and the maximum specific hydrogen production rate 23 5mL·(h·g)-1 were obtained at Fe concentration of 1000mg·L-1 and 500mg·L-1 . At the same concentration, the enhancement of Fe is better than Fe2+ when the concentration is higher than 50 mg·L-1. A sequential extraction technique was used to represent arbitrary divisions between different metal forms of Fe2+ in the mixed culture. |