| 化感物质对硝酸还原酶活性影响的研究 |
| 摘要点击 2778 全文点击 2347 投稿时间：1998-04-06 |
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| 中文关键词 化感物质 硝酸还原酶 抑制 促进 |
| 英文关键词 allelochemicals nitrate reductases inhibition stimulation |
| DOI 10.13227/j.hjkx.19990121 |
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| 中文摘要 |
| 3种来自小麦秸杆腐解过程中产生的化感物质(阿魏酸、苯甲酸、对羟基苯甲酸),在不同浓度及不同pH条件下对硝酸还原酶活性的影响实验结果表明,阿魏酸(t-FA)在0.26、2.58、5.15mmol/L浓度下对硝酸还原酶活性均表现出一定的抑制作用,抑制率分别为0.52%,11.6%和9.02%;苯甲酸(BA)在4.09、8.19mmol/L浓度下对硝酸还原酶的活性有抑制作用,其抑制率分别为18.4%和6.98%,在0.41mmol/L浓度下则表现出促进作用,促进率为3.07%;对羟基苯甲酸(p-HA)在0.36、1.81、3.62mmol/L浓度下对硝酸还原酶活性均表现出促进作用,促进率分别为6.86%、15.8%和8.73%,其中以1.81mmol/L浓度促进作用最强。阿魏酸,苯甲酸在不同pH下(pH6、pH7、pH8)对硝酸还原酶活性均表现出一定的抑制作用,其中pH6最为明显。阿魏酸在pH6时抑制率为11.6%,苯甲酸的抑制率为18.4%.阿魏酸、苯甲酸和对羟基苯甲酸3种化感物质的混合液,在pH6时对硝酸还原酶活性表现出显着的促进作用,促进率为12.7%,在pH7和pH8条件下则表现出抑制作用,抑制率分别为5.17%和12.4%.实验还表明NO2-的生成与N3-的转化之间是互相关联的。 |
| 英文摘要 |
| This paper described the research results on influence of trans ferulic acid (t-FA),benzoic acid (BA) and p hydroxybenzoic acid (p-HA) isolated from decomposition of wheat straw on the activity of nitrate reductases in different concentration and different pH. The results showed that the activity of nitrate reductases was inhibited by t FA under the concentration of 0.26, 2.58 and 5.15 mmol/L, and BA under the concentration of 4.09 and 8.19 mmol/L, the inhibition rate was 0.52%, 11.6%, 9.02%,18.4%,and 6.98%,respectively.0.41 mmol/L of BA, however, displayed some stimulation to nitrate reductases, the stimulation rate was 3.07%. The activity of nitrate reductases was stimulated by p HA under the concentration of 0.36, 1.81,3.62mmol/L, the stimulation rate was 6.86%, 15.8% and 8.73%, respectively. The more strong stimulation was observed at 1.81 mmol/L of p HA. The effect of different pH values on the activity of nitrate reductases was also studied. pH 6 for t FA and BA showed a obvious inhibition effect, the inhibition rate was 11.6% and 18.4%, respectively. The mixture of t FA,BA and p HA at pH6 showed a significant stimulation to nitrate reductases and inhibition effect at pH7 and pH8. between production of NO2-N and residue of NO3-N there was relationship. |
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