| 杀灭菊酯的微生物降解及酶促降解 |
| 摘要点击 2218 全文点击 2452 投稿时间：1996-10-02 |
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| 中文关键词 农药 微生物降解 酶促降解 杀灭菊酯 |
| 英文关键词 fenvalerate biodegradation enzymatic degradation |
| DOI 10.13227/j.hjkx.19970202 |
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| 中文摘要 |
| 测定了降解菌YF11及其胞内酶对杀灭菊酯的降解特性。在纯培养系统中,菌量为OD415nm0.20时,YF11对大于30mg/L的杀灭菊酯的降解呈零级动力学特征,浓度为30-100mg/L时,降解速率为1.876-2.124mg(Lh);浓度大于200mg/L的杀灭菊酯对降解菌YF11的降解产生抑制作用。降解速率与菌量近似成正比关系;10mg/L杀灭菊酯的降解曲线呈一级动力学特征。从YF11提取的粗酶液在32.5℃、pH8.0时对杀灭菊酯显示最大的降解活性,其最大降解速率为211.8nmol/(min·mg蛋白)、米氏常数(Km)为41.4nmol/ml。 |
| 英文摘要 |
| Degradation characteristics of fenvalerate by the isolated strain YF11 and its enzyme was determined.At biomass of 0.20(OD415nm)in pure culture system,degradation kinetics of >30mg/L of fenvalerate was considered to fit zero order model,their rates were range of 1.876 to 2.124mg/(L·h).The rate was obviously inhibited by >200mg/L of fenvalerate.It was found that the degradation rates of fenvalerate had a similar direct relationship to the biomass of strain YF11 at same concentration of fenvalerate.The degradation curve at 10mg/L of fenvalerate was considered to fit first-order model.Optimum activity of cell free enzyme from strain YF11 occurred at condition of 32.5℃ and pH 8.0.The maximum enzymatic degradation rate of fenvalerate and its Km were 211.8nmol/(min·mg)of protein and 41 4nmol/ml,respectively. |
