| 磁场对紫色非硫光合细菌脱氢酶活性的影响 |
| 摘要点击 1932 全文点击 1494 投稿时间：1994-12-02 |
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| 中文关键词 磁场 磁生物效应 紫色非硫光合细菌 固定化细胞 脱氢酶 |
| 英文关键词 magnetic field magnetic biological effect purple nonsulfur photosynthetic bacteria immobilized cells dehydrogenase |
| DOI 10.13227/j.hjkx.19950302 |
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| 中文摘要 |
| 研究并比较磁场作用下紫色非硫光合细菌(PSB)自然细胞和固定化细胞的某些性质。结果表明在最佳磁场作用下2种细胞的热稳定性、pH稳定性良好。与不加磁场一样,最适温度为30℃—40℃,最适pH值为8,但自然细胞脱氢酶活力比对照组提高10%—20%,固定化细胞提高20%—30%。磁场使细胞对金属离子(Fe2+、Mn2+、Mg2+)敏感性增加。2种细胞最佳磁场值不同,固定化细胞在最佳磁场作用下在4℃冰箱中保存45—210d脱氢酶活性稳定,仍可提高20%,在无磁场的同等条件下固定化细胞比自然细胞脱氢酶活力高1.5—2倍,显示出PSB细胞固定化处理的优越性。 |
| 英文摘要 |
| Some properties of native and immobilised cells of purple nonsulfur photosynthetic bacteria (PSB) were studied and compared under the condition of magnetic field. The results show that under the condition of optimum magnetic field both types of cells were thermally and PH stable, with an optimum dehydrogenase temperature of 30—40℃, and an optimum pH of 8. As compared to the control, the dehydrogenase activity was 10%—20% hig-her for native cells and was 20%—30% higher for immobilized cells. The magnetic. Field augmented the sensitivity of PSB to metal inos (Fe2+, Mn2+, Mg2+). But these two types of cells were different in the value of optimum magnetic field and the accumulative effect of magnetic field. The effect of magnetic field on the dehydrog6nase activity of immobilized cells remained constant after 45—210 days of storage at 4℃,with a dehydrogenase activity improved by 20%.Immobilized cells had a dehydrogenase activity of 150%—200% higher than native cells under the same conditions but without an applied mgnetic field. |
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