| 基于多个扩增子的DNA metabarcoding技术探究黄海微型真核浮游植物多样性 |
| 摘要点击 1500 全文点击 729 投稿时间：2018-11-04 修订日期：2019-03-22 |
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| 中文关键词 微型真核浮游植物 DNA metabarcoding ITS 18S rDNA V4 18S rDNA V9 多样性 |
| 英文关键词 eukaryotic nano-phytoplankton DNA metabarcoding ITS 18S rDNA V4 18S rDNA V9 biodiversity |
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| 中文摘要 |
| 微型真核浮游植物是海洋生态系统中能量流动和物质循环的关键环节，对维持海洋生态系统的稳定发挥着关键的作用.本研究首次应用DNA metabarcoding技术探讨黄海微型真核浮游植物的物种组成及多样性，对比分析ITS、18S rDNA V4和18S rDNA V9扩增子条件下微型真核浮游植物的群落结构及多样性水平，并探讨了其多样性与温度、盐度的响应关系.结果表明：①基于ITS扩增子获得的微型真核浮游植物中绿藻门所占比例较高，而基于18S rDNA V4和18S rDNA V9扩增子获得的甲藻门相对丰度较高.②基于18S rDNA V9扩增子获得的微型真核浮游植物的ACE、Chao1、Shannon和Simpson指数较ITS和18S rDNA V4扩增子更高，而且其门、纲、目和科的数目较高.③黄海微型真核浮游植物的Shannon指数与温度呈现出显著正相关的关系（P<0.01），而与盐度呈现出显著负相关的关系（P<0.05）.本研究通过对比不同扩增子条件下黄海微型真核浮游植物的群落结构及丰度特征，丰富了黄海微型真核浮游植物的认识，可为今后该海域微型真核浮游植物的生态学研究、生物多样性监测、生物资源动态变化及持续开发生产等方面的分析提供科学依据. |
| 英文摘要 |
| Eukaryotic nano-phytoplankton plays an indispensable role in energy flow, material circulation, and stability maintenance of marine ecosystems. In this research, DNA metabarcoding technology was applied to elucidate the distribution features of eukaryotic nano-phytoplankton in the Yellow Sea for the first time. The community structure and diversity of eukaryotic nano-phytoplankton were compared based mainly on amplicons of internal transcribed spacer (ITS), 18S rDNA V4 and 18S rDNA V9. Analysis of the correlation between diversity and temperature/salinity was performed as well. The following results were noted. ① Higher proportions of Chlorophyta were obtained based on the ITS region compared with other two amplicons, while the relative abundance of Dinoflagellata was larger when 18S rDNA V4 and 18S rDNA V9 regions were applied.②The abundance-based coverage estimators (ACE), Chao1, Shannon diversity, and Simpson diversity indices were significantly higher based on the amplicon of 18S rDNA V9 than those based on ITS and 18S rDNA V4. The numbers of phyla, classes, order, and families were larger when the 18S rDNA V9 region was applied.③ Based on the amplicons of ITS, 18S rDNA V4, and 18S rDNA V9, the Shannon diversity index showed a significant positive correlation with temperature (P<0.01), indicating that as the temperature increased, the diversity of the eukaryotic nano-phytoplankton increased. The tendency was opposite for salinity, however. With an increase in salinity, the Shannon diversity index decreased significantly (P<0.05). This research compared the community structure and diversity of eukaryotic nano-phytoplankton under different amplicons and will provide a scientific basis for ecological studies of eukaryotic nano-phytoplankton, monitoring of biodiversity and dynamic changes of biological resources, and sustainable development. |
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