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牛粪堆肥系统环境因子对抗性基因的影响
摘要点击 1683  全文点击 686  投稿时间:2018-12-22  修订日期:2019-01-09
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中文关键词  牛粪  抗性基因  堆肥  四环素  大环内酯  环境因子
英文关键词  cow manure  resistance genes  composting  tetracyclines  macrolides  environmental factors
作者单位E-mail
彭晶 哈尔滨工业大学环境学院, 哈尔滨 150090
哈尔滨工业大学建筑设计研究院, 哈尔滨 150090 
51609060@qq.com 
王科 哈尔滨工业大学环境学院, 哈尔滨 150090 hitwk@sina.com 
谷月 哈尔滨工业大学环境学院, 哈尔滨 150090  
王爱杰 哈尔滨工业大学环境学院, 哈尔滨 150090  
中文摘要
      本研究利用实时定量PCR技术检测牛粪60 d好氧堆肥过程中典型四环素类抗性基因(tetQ、tetW、tetM、tetG、tetAtetX)和大环内酯类抗性基因(erm35、erm36、ermB、ermF、ermTermX)的数量变化规律,系统研究了温度、含水率、挥发性有机质(VS)含量、碳氮比(C/N)、pH值、氧化还原电位(ORP)等堆肥系统环境因子和细菌数量(TCB)对这两类典型抗生素抗性基因的影响.结果表明,堆肥升温阶段牛粪中tetQ、tetW、tetGtetX数量分别增加1.7、0.3、84.8和4.5倍,而tetM丰度降低了83.1%;堆肥腐熟阶段牛粪中tetGtetX数量分别增加23.8和11.5倍,而tetW、tetQtetM数量均降低90%.整个牛粪堆肥过程中erm35、erm36、ermB、ermF、ermTermX丰度分别增加2.1、430.4、0.6、11.5、2.1和49.1倍.RDA分析表明,牛粪堆肥过程中对四环素与大环内酯类抗性基因数量影响较大的3个环境因子分别为:ORP(54.8%)、温度(34%)和VS(11.3%),其中ORP与ermXermB、温度与tetQermF以及VS与tetW数量均呈明显地正相关.
英文摘要
      The effects of environmental factors and bacterial numbers on typical tetracycline resistance genes (tetQ, tetW, tetM, tetG, tetA, and tetX) and macrolide resistance genes (erm35, erm36, ermB, ermF, ermT, and ermX) during cow manure composting were investigated and quantified via real-time quantitative PCR. The results suggested that the abundance of tetQ, tetW, tetG, and tetX increased by 1.7, 0.3, 84.8, and 4.5 times respectively, whereas tetM decreased by 83.1% during the heating stages of cow manure composting. The abundance of tetG and tetX increased by 23.8 and 11.5 times respectively, whereas tetW, tetQ, and tetM decreased by 90% during the maturity stage. However, the macrolide resistance genes (erm35, erm36, ermB, ermF, ermT, and ermX) increased by 2.1, 430.4, 0.6, 11.5, 2.1, and 49.1 times, respectively. Redundancy analysis showed that ORP, temperature, and VS were crucial factors that could explain the variations of 54.8%, 34%, and 11.3%, respectively, in ARGs. ORP, temperature, and VS also had the greatest positive correlation with ermX, ermB, tetQ, ermF, and tetW, in that order.

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