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室温低氨氮基质单级自养脱氮颗粒污泥启动效能与污泥特性
摘要点击 2039  全文点击 509  投稿时间:2018-08-07  修订日期:2018-09-14
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中文关键词  颗粒污泥  部分亚硝化-厌氧氨氧化  室温  污泥形态  胞外聚合物(EPS)
英文关键词  granular sludge  partial nitritation and ANAMMOX  room temperature  sludge morphology  extracellular polymeric substances(EPS)
作者单位E-mail
谢璐琳 苏州科技大学环境科学与工程学院, 苏州 215009 xielulin@outlook.com 
王建芳 苏州科技大学环境科学与工程学院, 苏州 215009
苏州科技大学天平学院, 苏州 215009
城市生活污水资源化利用技术国家地方联合工程实验室, 苏州 215009 
wjf302@163.com 
钱飞跃 苏州科技大学环境科学与工程学院, 苏州 215009
城市生活污水资源化利用技术国家地方联合工程实验室, 苏州 215009
江苏高校水处理技术与材料协同创新中心, 苏州 215009 
 
张泽宇 苏州科技大学环境科学与工程学院, 苏州 215009  
沈耀良 苏州科技大学环境科学与工程学院, 苏州 215009
城市生活污水资源化利用技术国家地方联合工程实验室, 苏州 215009
江苏高校水处理技术与材料协同创新中心, 苏州 215009 
 
齐泽坤 苏州科技大学环境科学与工程学院, 苏州 215009  
中文摘要
      室温低氨氮基质条件下单级自养脱氮工艺的启动和稳定运行是该工艺应用于市政污水处理的前提和基础.本研究在气升式反应器中接种久置的PN/A(partial nitritation and ANAMMOX)颗粒污泥,控制温度在(23±2)℃,pH在7.7~8.0,以氨氮浓度为70 mg·L-1的人工无机配水为基质,考察单级部分亚硝化-厌氧氨氧化实现室温启动效能.通过逐级缩短HRT(1.1 h→0.9 h→0.7 h→0.5 h)提升氮负荷[1.53 kg·(m3·d)-1→1.87 kg·(m3·d)-1→2.40 kg·(m3·d)-1→3.36 kg·(m3·d)-1],逐步恢复AOB、AMX菌活性以及微生物协同效能.经过95 d运行调控,反应器成功启动,NH4+-N和TN去除率达85%和69%.根据各阶段污泥性能,严格控制溶解氧,有效抑制NOB.污泥适应环境后,颗粒粒径随负荷提升逐渐增大,最终平均粒径达1.30 mm.成熟的自养颗粒污泥轮廓光滑清晰,扫描电镜显示,颗粒污泥内部形成空腔,表面有孔隙,污泥形态以球菌为主,并有少量杆菌及短杆菌.EPS主要成分为蛋白质(81.48%),泥水分离效果较好.
英文摘要
      The start-up and stable operation of single-stage autotrophic nitrogen removal process under low ammonia nitrogen substrate at room temperature appears as the premise and basis for the application in municipal wastewater treatment. In this study, the PN/A (partial nitritation and ANAMMOX) granular sludge for long-term storage was inoculated into an air-lift bioreactor to investigate the nitrogen removal performance during the start-up of single-stage partial nitritation and ANAMMOX process under the following conditions:temperature at (23±2)℃, pH at 7.7-8.0. Synthetic wastewater with ammonia nitrogen concentration of 70 mg·L-1 was used as influent. By stepwise shortening hydraulic retention time (HRT) (1.1 h→0.9 h→0.7 h→0.5 h) and increasing ammonia nitrogen loading rate[1.53 kg·(m3·d)-1→1.87 kg·(m3·d)-1→2.40 kg·(m3·d)-1→3.36 kg·(m3·d)-1], the bioactivity as the synergy between the ammonia oxidizing bacteria (AOB) and anaerobic oxidizing bacteria (AMX) were gradually restored. After 95 d operation and regulation, the process was successfully established and the removal rate of NH4+-N and TN were 85% and 69%, respectively. According to the performance of sludge at each stage, the nitrite oxidizing bacteria (NOB) were selectively inhibited by controlling dissolved oxygen strictly. The average particle size gradually increased and finally was reached to 1.30 mm after the sludge was adapted to the environment. The profile of the mature autotrophic granular sludge was smooth and clear, SEM showed that the center of granular sludge formed a cavity with porous structure on the surface, the sludge morphology consisted mainly of cocci, with a small amount of bacilli and short bacilli. The major component of EPS in granular sludge was protein (81.48%) indicating that it had a good settling performance.

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