首页  |  本刊简介  |  编委会  |  投稿须知  |  订阅指南  |  联系我们  |  微信  |  出版道德声明  |  Ei收录本刊数据
某集约化肉鸡饲养场PM2.5中抗生素抗性基因的分布特征
摘要点击 245  全文点击 78  投稿时间:2018-08-02  修订日期:2018-08-27
查看HTML全文 查看全文  查看/发表评论  下载PDF阅读器
中文关键词  肉鸡饲养场  PM2.5  抗生素抗性基因  粪便  一类整合子
英文关键词  broiler feeding operation  PM2.5  antibiotic resistance genes  feces  class I integrin
作者单位E-mail
刘菲 常州大学环境与安全工程学院, 常州 213164 ly_fhy@126.com 
许霞 常州大学环境与安全工程学院, 常州 213164  
屠博文 常州市疾病预防控制中心, 常州 213022  
汪楚乔 常州大学环境与安全工程学院, 常州 213164  
江晓栋 常州大学环境与安全工程学院, 常州 213164  
王利平 常州大学环境与安全工程学院, 常州 213164 wlpcczu@163.com 
薛银刚 常州大学环境与安全工程学院, 常州 213164
江苏省常州环境监测中心, 江苏省环境保护水环境生物监测重点实验室, 常州 213001 
yzxyg@126.com 
中文摘要
      集约化家禽饲养场是抗生素抗性基因(ARGs)的重要来源,而PM2.5作为ARGs可能向人体暴露的重要途径还未得到很好地研究.本文采集了集约化肉鸡饲养场舍内PM2.5和粪便以及舍外PM2.5样品,利用荧光定量PCR(qPCR)进行一类整合子(intI1)、总细菌(16S rDNA)和6类共19种ARGs丰度的检测.结果显示,除blaGES-1blaSHV-1之外,其余17种ARGs在6类样品中均有检出.磺胺类、四环素类、大环内酯类和氨基糖苷类抗性基因在舍内粪便中丰度较高,达到1.04×109~3.27×1010 copies·g-1,粪便是饲养场PM2.5中ARGs的主要来源.舍内PM2.5中以磺胺类和大环内酯类抗性基因丰度较高,分别为(8.9±1.9)×107 copies·m-3和(5.6±3.1)×107 copies·m-3,且舍内PM2.5中ARGs丰度明显高于舍外.PM2.5质量浓度与16S rDNA、intI1和ARGs丰度呈显著正相关,表明集约化饲养场中PM2.5是空气传播细菌、ARGs和intI1的储存库和传播者.6类样品中intI1丰度均高于ARGs,同时intI1和ARGs的共存关系表现出了多药耐药性的威胁,易对饲养人员和家禽健康及周边空气环境造成危害.
英文摘要
      Concentrated poultry feeding operations are an important source of antibiotic resistance genes (ARGs). Little attention has been given to PM2.5 as a mechanism for exposing ARGs to humans. In this study, PM2.5 and fecal samples from inside the broiler feeding operation and PM2.5 samples from outside the broiler feeding operation were collected. All samples were subjected to the determination of class Ⅰ integrin (intI1), total bacterial gene (16S rDNA), and 19 ARGs of six types by quantitative real-time PCR (qPCR). The results indicated that, excluding blaGES-1 and blaSHV-1, the remaining 17 ARGs were detected in all six samples. Sulfonamide resistance genes, tetracycline resistance genes, macrolide resistance genes, and aminoglycoside resistance genes were abundant in the feces, reaching 1.04×109-3.27×1010 copies·g-1, while feces was an important source of antibiotic resistance genes in PM2.5 of the broiler feeding operation. There were high abundances of sulfonamide resistance genes and macrolide resistance genes in PM2.5 from inside the broiler feeding operation, reaching (8.9±1.9)×107 copies·m-3 and (5.6±3.1)×107 copies·m-3, respectively. The abundance of ARGs in the PM2.5 samples from inside the broiler feeding operation was significantly higher compared to the outside PM2.5 samples. There was a significant positive correlation between PM2.5 mass concentration and 16S rDNA, intI1, and ARGs abundance, indicating that PM2.5 was the reservoir and disseminator of airborne bacteria, ARGs, and intI1 in the broiler feeding operation. The abundance of intI1 was higher than ARGs among all samples, and the co-occurring relationship between intI1 and ARGs demonstrates the threat of multi-drug resistance, which is harmful to the surrounding air environment and the health of the breeder and poultry.

您是第15235484位访客
主办单位:中国科学院生态环境研究中心 单位地址:北京市海淀区双清路18号
电话:010-62941102,62849343 传真:010-62849343 邮编:100085 E-mail: hjkx@rcees.ac.cn
本系统由北京勤云科技发展有限公司设计  京ICP备05002858号