| 高温堆肥过程对猪粪来源抗生素抗性基因的影响 |
| 摘要点击 2721 全文点击 1317 投稿时间：2015-10-27 修订日期：2015-12-15 |
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| 中文关键词 猪粪 堆肥 抗生素抗性基因 erm bla qnr 荧光定量PCR |
| 英文关键词 swine manure composting antibiotic resistance genes erm bla qnr real-time quantitative PCR |
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| 中文摘要 |
| 为研究大规模好氧高温堆肥过程对猪粪来源抗生素抗性基因的影响,采用荧光定量PCR法检测了4个大环内酯类抗性基因(erythromycin resistance methylase, ermA、ermB、ermC 和ermF)、3个β-内酰胺抗性基因(beta-lactamase, blaTEM、blaCTX和blaSHV)和2个喹诺酮类抗性基因(quinolone resistance, qnrA和qnrS)在堆肥过程中的变化趋势.结果表明,堆肥初期大环内酯类抗性基因含量显著高于β-内酰胺类和喹诺酮类抗性基因(P<0.01),其中ermB基因丰度最高为9.88×108 copies·g-1,其次是ermF为9.40×108 copies·g-1.在高温堆肥结束时β-内酰胺抗性基因和喹诺酮类抗性基因丰度维持在较低水平,而大环内酯类抗性基因在堆肥结束后仍然具有较高含量.其中,ermF基因与堆肥初期相比甚至出现了增加的情况,这表明高温堆肥过程不能有效去除所有抗生素抗性基因.对于某些抗生素抗性基因,堆肥可能还是良好的生物反应器而导致抗性基因的增殖,农田中堆肥产品的施用有可能会造成抗生素抗性基因的传递. |
| 英文摘要 |
| To investigate the effects of thermophilic composting process on antibiotic resistance genes (ARGs) of swine manure source at a field scale, the abundance of four erythromycin resistance genes (ermA, ermB, ermC and ermF), three β-lactam resistance genes(blaTEM, blaCTX and blaSHV) and two quinolone resistance genes(qnrA and qnrS) were quantified by quantitative PCR(qPCR) during the composting process. The results suggested that the erm genes' copy numbers were significantly higher than those of the bla and qnr genes in the early stage of composting (P<0.01). The maximum abundance of erm genes was ermB (9.88×108 copies·g-1), following by ermF(9.4×108 copies·g-1). At the end of the composting process, bla and qnr genes were at low levels, while erm genes were still at high levels. Even through ermF was proliferated comparing with the initial copies. These results indicated that thermophilic composting process could not effectively remove all ARGs. For some ARGs, compost may be a good bioreactor resulting in their proliferation. Application of composting products on farmland may cause transference of ARGs. |
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