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外源硒对黄瓜抗性、镉积累及镉化学形态的影响
摘要点击 3318  全文点击 1317  投稿时间:2014-07-07  修订日期:2014-08-16
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中文关键词    抗氧化酶  丙二醛  镉累积  镉形态  黄瓜
英文关键词  selenium  antioxidant enzymes  malonaldehyde  Cd accumulation  Cd forms  cucumber
作者单位E-mail
熊仕娟 西南大学资源环境学院, 重庆 400716 xiongshijuan5@163.com 
刘俊 西南大学资源环境学院, 重庆 400716  
徐卫红 西南大学资源环境学院, 重庆 400716 xuwei_hong@163.com 
谢文文 西南大学资源环境学院, 重庆 400716  
陈蓉 西南大学资源环境学院, 重庆 400716  
张进忠 西南大学资源环境学院, 重庆 400716  
熊治庭 武汉大学资源与环境科学学院, 武汉 430079  
王正银 西南大学资源环境学院, 重庆 400716  
谢德体 西南大学资源环境学院, 重庆 400716  
中文摘要
      采用盆栽试验,在土壤Cd(20 mg·kg-1)污染条件下,测定了不同浓度的Na2SeO3(Se水平为0、0.5和1.0 mg·L-1)处理对2个黄瓜(Cucumis satiuus L.)品种(低Cd富集植物品种津优1号和高Cd富集品种燕白)生物量、丙二醛(MDA)含量、抗氧化酶活性及黄瓜体内Cd形态和Cd积累量的影响. 结果表明,叶面喷施硒(Na2SeO3)后,黄瓜叶、茎、根、果实、植株干重,以及Cd含量和积累量在两个品种间的差异达到了显著性水平. 随硒浓度的增加,燕白叶的丙二醛(MDA)含量逐渐增加,津优1号叶的丙二醛含量则逐渐减少; 燕白根的丙二醛含量先增后降,津优1号根的丙二醛含量先降后增. 喷施Na2SeO3后,黄瓜叶和根的CAT、SOD和POD活性呈现不同变化趋势. 喷施外源硒降低了2个品种果实中不同形态Cd含量. 叶面喷施Na2SeO3使黄瓜叶、茎、根和果实中的Cd含量分别下降了 3.2%~17.9%、14.6%~28.2%、5.1%~18.5%和60.6%~75.8%. 比较2个供试黄瓜品种,无论喷施Na2SeO3与否,黄瓜植株中Cd积累量以津优1号>燕白.
英文摘要
      Pot experiments were carried out to investigate the influence of different selenium(Se)levels(0, 0.5 and 1.0 mg·L-1) on the plant growth, concentration of malonaldehyde(MDA), activities of antioxidant enzymes, accumulation and chemical forms of cadmium(Cd)in cucumber when exposed to Cd(20 mg·kg-1). The results showed that dry weights of leaf, stem, root, fruit and plant, and concentrations and accumulation of Cd significantly differed between two varieties of cucumber Yanbai and Jinyou 1. With increasing levels of Se, the contents of MDA in the leaves of Yanbai increased, but the contents of MDA in the leaves of Jinyou 1 decreased. The contents of MDA in the roots of Yanbai first increased and then decreased, while the contents of MDA in Jinyou 1 first decreased and then increased. The variation trends of CAT, SOD and POD in the leaves and roots of both varieties were different with increasing levels of Se. The concentrations of different chemical forms of Cd in the fruit decreased after spraying of Se, compared with the control. Cadmium concentrations in the leaves, stem, roots and fruit of both varieties decreased by 3.2%-17.9%, 14.6%-28.2%, 5.1%-18.5% and 60.6%-75.8% in the presence of Se when exposed to Cd. Accumulation of Cd in the plant of both varieties was in order of Jinyou 1>Yanbai in the presence or absence of Na2SeO3.

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