| 微生物群落对土壤微生物呼吸速率及其温度敏感性的影响 |
| 摘要点击 2576 全文点击 1509 修订日期：2011-06-20 |
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| 中文关键词 微生物群落 土壤呼吸 温度敏感性 PCR-DGGE Q10 |
| 英文关键词 microbial community soil respiration temperature sensitivity PCR-DGGE Q10 |
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| 中文摘要 |
| 通过灭菌和重新接种不同土壤微生物群落的方法,探索了改变土壤微生物群落对土壤微生物呼吸速率及其温度敏感性的影响.结果表明,灭菌海伦黑土接种未灭菌的哈尔滨黑土、封丘潮土和祁阳红壤后, 15℃时土壤CO2的累积释放量分别为684.25、753.97和644.91 μg, 25℃ 时土壤CO2的累积释放量分别为963.06、1015.44和852.31 μg, 35℃ 时土壤CO2的累积释放量分别为1252.55、380.36和1177.88 μg; 土壤CO2的累积释放量随接种剂土壤pH增加而增加,而与接种剂土壤有机质含量高低、接种剂土壤与被接种土壤的地理距离无关.15、25和35℃ 时,接种不同微生物群落的土壤呼吸速率表现差异的持续时间分别为104、277和1177 h,差异持续时间随培养温度增高而延长.接种未灭菌的哈尔滨黑土、封丘潮土和祁阳红壤后,土壤呼吸温度敏感性指数Q10在0~104 h分别为1.63、1.49和1.80,在0~277 h分别为1.43、1.39和1.46,在0~1609 h分别为1.35、1.35和1.35; 呼吸温度敏感性随接种剂土壤pH降低而增加,随培养时间延长而减小.本研究表明改变土壤微生物群落将影响土壤微生物呼吸速率及其温度敏感性. |
| 英文摘要 |
| Inoculating common sterilized soil with different non-sterilized soils was used to investigate the potentials of altered microbial communities to change rate and temperature sensitivity of soil microbial respiration. Results showed that accumulative CO2 emission of sterilized Hailun black soils inoculated with non-sterilized Harbin black soil, Fengqiu fluvo-aquic soil and Qiyang red soil were 684.25, 753.97 and 644.91 μg, respectively, at 15℃; were 963.06, 1015.44 and 852.31 μg, respectively, at 25℃; and were 1252.55, 380.36 and 1177.88 μg, respectively, at 35℃. The soil accumulative CO2 emissions increased with pH of inoculant soils, but did not relate to organic content of and geographical distance from inoculant soils. Difference of microbial respiration rates among the three inoculant soils kept for 104, 277 and 1177 h at 15℃, 25℃ and 35℃, respectively. Temperature sensitivity quotients (Q10) of microbial respiration of sterilized Hailun black soils inoculated with non-sterilized Harbin black soil, Fengqiu fluvo-aquic soil and Qiyang red soil were 1.63, 1.49 and 1.80, respectively, during 0-104 h; were 1.43, 1.39 and 1.46, respectively, during 0-277 h and were 1.35, 1.35 and 1.35, respectively, during 0-1609 h. The Q10 decreased with pH of inoculant soils and incubation time. The results suggest that altered microbial community would change rate and temperature sensitivity of soil microbial respiration. |
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