| 海洋浮游细菌在东海赤潮高发区的分布与活性 |
| 摘要点击 3063 全文点击 2163 投稿时间：2009-03-19 修订日期：2009-05-26 |
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| 中文关键词 赤潮 东海 浮游细菌 胞外酶活性 细菌群落结构 |
| 英文关键词 harmful algae blooms (HABs) East China Sea bacterioplankton extracellular enzyme activity (EEA) bacterial community structure |
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| 中文摘要 |
| 于2006年4～5月对东海近海28°～30.7°N之间的赤潮高发区浮游细菌的分布、活性和群落结构特征进行了调查.采用荧光显微计数法测定细菌丰度，荧光模拟底物法测定细菌胞外酶活性， PCR-DGGE分析该区域的细菌群落结构特征.结果表明，东海赤潮高发区的浮游细菌丰度在5.85×104～9.26×105cells·mL-1之间，其中舟山外海区域较高，在整个调查区域北部海域高于南部海域.β-葡萄糖苷酶的平均活性为0.023 μmol |
| 英文摘要 |
| The distribution, activity and community structure of bacterioplankton in surface water were investigated at frequent harmful algae blooms (HABs) area in East China Sea (28°-30.7°N) from April to May, 2006. The abundance of bacterioplankton was determined by using the DAPI (4, 6-diamidino-2-phenylindole) direct count (DDC) method. The β-glucosidase and aminopeptidase activity were measured with fluorogenic model substrates. And the bacterial community structure was analyzed by PCR-DGGE. Results showed that bacterial abundance in northern of the sampling area was much more than that in southern of the sampling area. It ranged from 5.85×104 cells·mL-1 to 9.26×105cells·mL-1. And there was the highest value area outer the costal of Zhou Shan Island. The average aminopeptidase activity was 3.6 times of β-glucosidase activity which was 0.023 μmol·(L·h)-1 in this area. The β-glucosidase activity in > 5μm fraction contributed 47.4％ of the total, and that of the aminopeptidase activity was 44.24％ of the total. Bacterial extracellular enzyme activity had a higher average value in southern of the sampling area. This indicated that the bacterial activity had no direct relationship with bacterial abundance. Bacterial diversity and community structure differed from each sampling station. There were more divers in northern sampling area. The results suggested that the human being activity and continental inputting organic matters played a key role on the distribution of bacterial abundance. The distribution of bacterial extracellular enzyme activity was mainly affected by the Taiwan warm current. And it was the complicated unknown factors that caused the difference of the bacterial community structure and diversity from each sampling station. Obviously, it needed further work to enhance the knowledge of the ecological function of the bacterioplankton at frequent HABs area of the coastal water in East China Sea. |
